Thermacetogenium phaeum gen. nov., sp. nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium.

A novel anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium, strain PB(T), was isolated from a thermophilic (55 degrees C) anaerobic methanogenic reactor which had been treating kraft-pulp waste water. The bacterium oxidized acetate in co-culture with a thermophilic hydrogenotrophic methanogen. Strain PB(T), a gram-positive, spore-forming, rod-shaped bacterium grew optimally at 58 degrees C and pH 6.8. The bacterium grew acetogenically on several alcohols, methoxylated aromatics, pyruvate, glycine, cysteine, formate and hydrogen/CO2. Strain PB(T) also oxidized acetate with reduction of sulfate or thiosulfate as the electron acceptor. The bacterium contained MK-7 as the major quinone. The G+C content of the DNA was 53.5 mol%. Comparative 16S rDNA analysis indicated that strain PB(T) belongs to the Bacillus-Clostridium subphylum. However, it was distant from any known genera or micro-organism. The closest known relative was Thermoterrabacterium ferrireducens with 87.4% similarity. The name Thermacetogenium phaeum gen. nov., sp. nov. is proposed. The type strain is strain PBT (= DSM 12270T).

[1]  Y. Kamagata,et al.  Acetogenesis from pyruvate by Desulfotomaculum thermobenzoicum and differences in pyruvate metabolism among three sulfate-reducing bacteria in the absence of sulfate , 1993 .

[2]  B. Schink,et al.  Clostridium ultunense sp. nov., a mesophilic bacterium oxidizing acetate in syntrophic association with a hydrogenotrophic methanogenic bacterium. , 1996, International journal of systematic bacteriology.

[3]  S. Goodison,et al.  16S ribosomal DNA amplification for phylogenetic study , 1991, Journal of bacteriology.

[4]  J. Tamaoka,et al.  Analysis of bacterial menaquinone mixtures by high performance liquid chromatography , 1983 .

[5]  T. Lien,et al.  Desulfotomaculum thermocisternum sp. nov., a Sulfate Reducer Isolated from a Hot North Sea Oil Reservoir , 1996 .

[6]  J. Wiegel,et al.  Isolation and characterization of the homoacetogenic thermophilic bacterium Moorella glycerini sp. nov. , 1997, International journal of systematic bacteriology.

[7]  J. Ferry,et al.  Methane from acetate , 1992, Journal of bacteriology.

[8]  P. Lawson,et al.  The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. , 1994, International journal of systematic bacteriology.

[9]  G. Ritter,et al.  A New Type of Glucose Fermentation by Clostridium thermoaceticum , 1942, Journal of bacteriology.

[10]  Stephen H. Zinder,et al.  Isolation and Characterization of a Thermophilic Bacterium Which Oxidizes Acetate in Syntrophic Association with a Methanogen and Which Grows Acetogenically on H2-CO2 , 1988, Applied and environmental microbiology.

[11]  Birgitte Kiær Ahring,et al.  Acetate oxidation in a thermophilic anaerobic sewage-sludge digestor: the importance of non-aceticlastic methanogenesis from acetate , 1991 .

[12]  A. Hiraishi,et al.  Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification , 1992, Letters in applied microbiology.

[13]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[14]  K. Komagata,et al.  Determination of DNA base composition by reversed-phase high-performance liquid chromatography , 1984 .

[15]  B. Schink.  Energetics of syntrophic cooperation in methanogenic degradation , 1997, Microbiology and molecular biology reviews : MMBR.

[16]  M. P. Bryant,et al.  Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO2 to Methanogenesis in Cattle Waste at 40 and 60°C , 1981, Applied and environmental microbiology.

[17]  Eiichi Mikami,et al.  Isolation and Characterization of a Novel Thermophilic Methanosaeta Strain , 1991 .

[18]  D. Mountfort,et al.  Changes in proportions of acetate and carbon dioxide used as methane precursors during the anaerobic digestion of bovine waste , 1978, Applied and environmental microbiology.

[19]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[20]  M. Dreier,et al.  Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring. , 1997, International journal of systematic bacteriology.

[21]  D. Lovley,et al.  Intermediary Metabolism of Organic Matter in the Sediments of a Eutrophic Lake , 1982, Applied and environmental microbiology.