Gastrointestinal Pathology: Poster#140 SEQUENCE ANALYSIS OF THE E-CADHERIN (CDH1) GENE IN A COHORT OF GASTRIC CANCERS SEEN IN THE WESTERN CAPE

Background: Germline and somatic mutations of the CDH1 (E-cadherin) gene play a significant role in the development of gastric cancer. Germline mutations are present in approximately 29–56% of familial gastric cancers. Somatic mutations are seen in about 31% of sporadic cancers and have been identified in exons 7 to 10, with exons 8 and 9 being the commonly mutated regions. Objective: To determine the prevalence of CDH1 exon 8 mutations in sporadic gastric cancers and to correlate these findings with demographic and clinicopathological data. Design: A total of 44 gastric cancer cases were used in this study. DNA was extracted and CDH1 exon 8 was amplified and subjected to sequence analysis. The sequencing data were then analysed using ClastalW and BioEdit bioinformatics tools. Results: DNA from exon 8 could be amplified in 26 of the 44 samples. CDH1 mutations were identified in two of the 26 (7.6%) cases. The nucleotide changes of the two cases did not change protein the structure. There was no significant correlation between mutations and clinicopathological features due to the low frequency of mutations in this study. Conclusion: Our study showed a low prevalence of CDH1 exon 8 mutations in this region.

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