Human adipose-derived stem cells can optimize the filling material in rats.

BACKGROUND Human adipose-derived stem cells have been identified as a promising candidate for cell-assisted therapy to improve graft survival. OBJECTIVE To objective of the study was to add human adipose-derived stem cells into filling materials. METHODS The filling materials were prepared and divided into 6 groups: fat particles with phosphate buffer saline or human adipose-derived stem cells; acellular dermal matrix particles with phosphate buffer saline or human adipose-derived stem cells; mixture of fat particles and acellular dermal matrix particles with phosphate buffer saline or human adipose-derived stem cells. The survival rate, vascular density and histological at 2, 6 and 12 weeks were investigated. RESULTS Human adipose-derived stem cells significantly improved survival rate in each group at 6 and 12 weeks, and it significantly increased the vascular density in the fat particles and porcine acellular dermal matrix combined group and porcine acellular dermal matrix group at three time points, but human adipose-derived stem cells did not have a significant effect in the fat particles group. CONCLUSION Human adipose-derived stem cells as assisted cells added into filling material can improve survival rate and vascular density in rats.

[1]  B. Cheng,et al.  Autogenous Fat Transplantation and Botulinum Toxin Injection Into the Masseter Muscle to Create an Ideal Oval Face. , 2021, Aesthetic surgery journal.

[2]  Jinghong Xu,et al.  Application of adipose-derived stem cells in photoaging: basic science and literature review , 2020, Stem cell research & therapy.

[3]  Qinke Yao,et al.  Human adipose-derived stem cells enriched with VEGF-modified mRNA promote angiogenesis and long-term graft survival in a fat graft transplantation model , 2020, Stem cell research & therapy.

[4]  L. Rochette,et al.  Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing , 2020, International journal of molecular sciences.

[5]  Jianda Zhou,et al.  A Potential Filling Material for Wound Healing and Shaping: Acellular Dermal Matrix Combined with Autologous Dermis , 2019, Aesthetic Plastic Surgery.

[6]  M. Longaker,et al.  Fat Chance: The Rejuvenation of Irradiated Skin , 2019, Plastic and reconstructive surgery. Global open.

[7]  C. A. Salzberg,et al.  The use of acellular dermal matrix in breast reconstruction: evolution of techniques over 2 decades. , 2019, Gland surgery.

[8]  A. Swift,et al.  Facial Assessment and Injection Guide for Botulinum Toxin and Injectable Hyaluronic Acid Fillers: Focus on the Upper Face , 2017, Plastic and reconstructive surgery.

[9]  S. Chung,et al.  Therapeutic Mechanisms of Human Adipose-Derived Mesenchymal Stem Cells in a Rat Tendon Injury Model , 2017, The American journal of sports medicine.

[10]  F. Simonacci,et al.  Autologous fat transplantation for breast reconstruction: A literature review , 2016, Annals of medicine and surgery.

[11]  C. Winslow Filling the Midface: Injectables , 2016, Facial Plastic Surgery.

[12]  I. Bazarov,et al.  Human acellular dermal wound matrix for treatment of DFU: literature review and analysis. , 2015, Journal of wound care.

[13]  J. Kastrup,et al.  Enrichment of autologous fat grafts with ex-vivo expanded adipose tissue-derived stem cells for graft survival: a randomised placebo-controlled trial , 2013, The Lancet.

[14]  A. Zomorodipour,et al.  Human adipose‐derived mesenchymal stem cells can survive and integrate into the adult rat eye following xenotransplantation , 2013, Xenotransplantation.

[15]  Min Zhu,et al.  Comparison of Three Different Fat Graft Preparation Methods: Gravity Separation, Centrifugation, and Simultaneous Washing with Filtration in a Closed System , 2013, Plastic and reconstructive surgery.

[16]  K. Yoshimura,et al.  The Fate of Adipocytes after Nonvascularized Fat Grafting: Evidence of Early Death and Replacement of Adipocytes , 2012, Plastic and reconstructive surgery.

[17]  Sean B. Bury,et al.  Acellular dermal matrix in the management of the burn patient. , 2011, Aesthetic surgery journal.

[18]  L. Liao,et al.  Mesenchymal-stem-cell-based experimental and clinical trials: current status and open questions , 2011, Expert opinion on biological therapy.

[19]  K. Yoshimura,et al.  Adipose-derived stem/progenitor cells: roles in adipose tissue remodeling and potential use for soft tissue augmentation. , 2009, Regenerative medicine.

[20]  D. Prockop,et al.  Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. , 2006, Cytotherapy.