Protein Extraction Using Reverse Micelles

The controlled solubilization of proteins in a reverse micelle‐containing organic phase is considered as a method for separation of proteins by liquid‐liquid extraction. Cytochrome‐C was transferred between a bulk aqueous phase and a micellar phase, using the Aerosol OT‐isooctane system of surfactant and solvent. It was observed that the extent of protein solubilization could be controlled by varying the ionic strength of the aqueous phase equilibrated with the micellar phase.

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