Application of Ultrasonic Waves as a Priming Technique for Accelerating and Enhancing the Germination of Barley Seed: Optimization of Method by the Taguchi Approach

In this study the effects of different intensities and exposure time of ultrasound on barley seed have been investigated to determine the optimal conditions for accelerating germination. For optimization, the Taguchi approach was used. The germination rates and/or yield of the treated sample seed were compared with those of the untreated seed germinated under normal conditions. The seeds were treated with ultrasonic energy (input power 20-90% of 460W) and were exposed to three time periods ranging from 5 to 15 min. It was found that germination of the treated barley seed was increased about 1.042-1.065 times compared with that of the untreated seed. The ultrasonic treatment proved to be efficient in reducing the germination period by 30-45%. It was concluded that priming of seeds with ultrasound was effective in improving water uptake and germination. The data presented in this paper suggest that the increase in final germination percentage, together with the reduced germination period in treated seeds, may be due to the action of ultrasound and that it led to an improved hydration process with concurrent shell fragmentation.

[1]  K. Eastwell,et al.  Effect of ethylene on the gibberellic Acid-enhanced synthesis and release of amylase by isolated barley aleurone layers. , 1982, Plant physiology.

[3]  S. Naseer,et al.  Effect of Salt Stress on Germination and Seedling Growth of Barley (Hordeum vulgare L.) , 2001 .

[4]  Timothy J. Mason,et al.  Quantifying sonochemistry: Casting some light on a ‘black art’ , 1992 .

[5]  A. G. Gordon The use of ultrasound in agriculture , 1963 .

[6]  S. Shimomura The effects of ultrasonic irradiation on sprouting radish seed , 1990, IEEE Symposium on Ultrasonics.

[7]  M. Vinatoru,et al.  Investigation of the effects of ultrasound on vegetal tissues during solvent extraction. , 2001, Ultrasonics sonochemistry.

[8]  L. Taiz,et al.  Gibberellic Acid enhancement of DNA turnover in barley aleurone cells. , 1977, Plant physiology.

[9]  K. Suslick Sonoluminescence and sonochemistry , 1997, 1997 IEEE Ultrasonics Symposium Proceedings. An International Symposium (Cat. No.97CH36118).

[10]  A. Aladjadjiyan Increasing carrot seeds (Daucus carota L.), cv. Nantes, viability through ultrasound treatment , 2002 .

[11]  J. Bewley,et al.  Protein Synthesis in the Axes of Polyethylene Glycol-Treated Pea Seed and during Subsequent Germination , 1989 .

[12]  H. Honsová,et al.  Barley seed sensitivity to water stress at germination stage , 2018 .

[13]  J. W. T. Mason,et al.  Chemistry with ultrasound , 1990 .

[14]  Malcolm J. W. Povey,et al.  Ultrasound in Food Processing , 1995 .

[15]  M. Becker,et al.  Seed priming enhances germination and seedling growth of barley under conditions of P and Zn deficiency , 2004 .

[16]  S. A. Hebling,et al.  Efeitos do ultra-som de baixa intensidade na germinação de sementes de milho (Zea mays L.) sob diferentes condições de disponibilidade hídrica , 1995 .

[17]  R. Ellis The quantification of ageing and survival in orthodox seeds , 1981 .

[18]  A. A. Powell,et al.  Evidence for Repair Processes in the Invigoration of Seeds by Hydration , 1984 .

[19]  J. Jacobsen,et al.  Cytochemical localization of phosphatase in barley aleurone cells: The pathway of gibberellic-acid-induced enzyme release , 2004, Planta.