论文信息 - Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy

Coherent white light confocal fluorescence imaging and fluorescence lifetime imaging microscopy

We report on the application of a simple white light source based on the supercontinuum generation from commercial photonic crystal fibres to confocal fluorescence microscopy and fluorescence lifetime imaging (FLIM) microscopy. The coherent white light can be tuned by varying the wavelength and intensity of the pump, a Ti:Sapphire laser. There are several advantages jn the use of SC sources: spatially coherent white radiation, tuning ranges of approximately 400 nm, high brightness, a robust compact system (potentially all-fibre) and relatively low cost. Being pulsed, SC sources are suitable for FLIM and may be used for multiple excitation.

[1] J. Lakowicz. Principles of fluorescence spectroscopy , 1983 .

[2] A. Stentz,et al. Visible continuum generation in air–silica microstructure optical fibers with anomalous dispersion at 800 nm , 2000 .

[3] P. Georges,et al. Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells. , 2007, Applied optics.

[4] C. Dunsby,et al. An electronically tunable ultrafast laser source applied to fluorescence imaging and fluorescence lifetime imaging microscopy , 2004, (CLEO). Conference on Lasers and Electro-Optics, 2005..

[5] Mary-Ann Mycek,et al. Time-resolved optical imaging provides a molecular snapshot of altered metabolic function in living human cancer cell models. , 2006, Optics express.

[6] P. Bastiaens,et al. Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. , 1999, Trends in cell biology.