ANALYSIS OF HUMAN GALLSTONES BY FOURIER TRANSFORM INFRARED (FTIR)

Objective: The present study was aimed at determining the composition of gallstones removed from patients in Southern Sindh, Pakistan. Methodology: One hundred nine (109) gallstone samples surgically removed from as many patients (98 females and 11 males; age range 20 to 80 years) admitted for treatment in Liaquat University hospital, Jamshsoro during 2000 to 2003, were analyzed for composition by Fourier Transform Infrared (FTIR) spectroscopy. Results: Seventy four (67.9%) of the 109 gallstone samples were found to be pure cholesterol stones, Five (4.6%) pure calcium carbonate stones, Thirteen (11.9%) cholesterol + calcium carbonate, Ten (9.2%) cholesterol + bilirubin and Seven (6.4%) calcium bilirubinate stones. In mixed composition gallstones cholesterol was concentrated more at periphery than in the center of stone. Cholesterol either singly (67.9%) or in combination with either calcium carbonate (11.9%) or bilirubin (9.2%) was the most predominant component of gallstones. Conclusion: Analysis of gallstones based on FTIR suggests that cholesterol either singly or in combination with either calcium carbonate or bilirubin is the most predominant component of gallstones in Southern Sindh, Pakistan.

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