Structural deviations in poly(amidoamine) dendrimers: a MALDI-TOF MS analysis

Abstract A step-by-step synthesis/purification (CC, HILIC, HPLC) of poly(amidoamine) PAMAM dendrimers was performed. MALDI-TOF MS in the linear and reflectron mode was used to analyze the purified samples and byproduct samples of G0–G5 generations of the dendrimers up to the mass of 35 000 Da. DHB/fucose was found to give the best resolution, causing the least fragmentation of the samples. The precise mass number for the ideally branched dendrimers and their “structural errors” was obtained. The profile of the structural errors was established.

[1]  A. Proctor,et al.  Improvement of signal reproducibility and matrix/comatrix effects in MALDI analysis , 1995 .

[2]  J. Stults,et al.  Tryptic mapping of recombinant proteins by matrix-assisted laser desorption/ionization mass spectrometry. , 1993, Analytical chemistry.

[3]  D. Tirrell,et al.  Chain dynamics in poly(amidoamine) dendrimers: a study of proton NMR relaxation parameters , 1992 .

[4]  M. Lopp,et al.  SYNTHESIS AND CZE ANALYSIS OF PAMAM DENDRIMERS WITH AN ETHYLENEDIAMINE CORE , 2001, Proceedings of the Estonian Academy of Sciences. Chemistry.

[5]  John. Mitchell.  Applied polymer analysis and characterization , 1987 .

[6]  M. Nielen.  MALDI TIME-OF-FLIGHT MASS SPECTROMETRY OF SYNTHETIC POLYMERS , 1999 .

[7]  Michael B. Hall,et al.  Dendritic macromolecules: synthesis of starburst dendrimers , 1986 .

[8]  P. Chaurand,et al.  Post-source decay and delayed extraction in matrix-assisted laser desorption/ionization-reflectron time-of-flight mass spectrometry. Are there trade-offs? , 1996, Rapid communications in mass spectrometry : RCM.

[9]  Lars T. Piehler,et al.  Slab-gel and capillary electrophoretic characterization of polyamidoamine dendrimers , 1998 .

[10]  M Vaher,et al.  Application of capillary zone electrophoresis to the separation and characterization of poly(amidoamine) dendrimers with an ethylenediamine core. , 2002, Journal of chromatography. A.

[11]  M. Lopp,et al.  FRAGMENTATION OF PAMAM DENDRIMERS IN METHANOL , 2001, Proceedings of the Estonian Academy of Sciences. Chemistry.

[12]  G. Kallos,et al.  Molecular weight determination of a polyamidoamine Starburst polymer by electrospray ionization mass spectrometry , 1991 .

[13]  Richard D. Smith,et al.  Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometric characterization of high molecular mass Starburst™ dendrimers , 1997 .

[14]  James R. Dewald,et al.  A New Class of Polymers: Starburst-Dendritic Macromolecules , 1985 .

[15]  R. Crooks,et al.  Characterization of Poly(amidoamine) Dendrimers and Their Complexes with Cu2+ by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry , 2001 .

[16]  Michael Karas,et al.  Matrix Dependence of Metastable Fragmentation of Glycoproteins in MALDI TOF Mass Spectrometry , 1995 .

[17]  William A. Goddard,et al.  Starburst Dendrimers: Molecular‐Level Control of Size, Shape, Surface Chemistry, Topology, and Flexibility from Atoms to Macroscopic Matter , 1990 .