Directed evolution of an esterase: screening of enzyme libraries based on pH-indicators and a growth assay.
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U. Bornscheuer | J. Altenbuchner | U T Bornscheuer | J Altenbuchner | H H Meyer | H. Meyer | J. Altenbuchner
[1] F. Arnold,et al. Directed evolution of enzyme catalysts. , 1997, Trends in biotechnology.
[2] C. Sih,et al. A chemoenzymatic synthesis of optically-active dihydropyridines , 1991 .
[3] I. Pelletier,et al. A bacterial esterase is homologous with non-haem haloperoxidases and displays brominating activity. , 1995, Microbiology.
[4] C. Sih,et al. Quantitative analyses of biochemical kinetic resolutions of enantiomers , 1982 .
[5] A. Greener,et al. An efficient random mutagenesis technique using an E. coli mutator strain. , 1996, Methods in molecular biology.
[6] F. Arnold. Design by Directed Evolution , 1998 .
[7] U. Bornscheuer,et al. Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones. , 1998, Biotechnology and bioengineering.
[8] H. Sobek,et al. The metal-ion-free oxidoreductase from Streptomyces aureofaciens has an α/β hydrolase fold , 1994, Nature Structural Biology.
[9] K. Achiwa,et al. Acyloxymethyl as an activating group in lipase-catalyzed enantioselective hydrolysis. A versatile approach to chiral 4-aryl-1,4-dihydro-2,6-dimethyl-3,5-pyridinedicarboxylates. , 1991 .
[10] R. Verger,et al. Lipases: Interfacial Enzymes with Attractive Applications. , 1998, Angewandte Chemie.
[11] W. Stemmer,et al. Searching Sequence Space , 1995, Bio/Technology.
[12] U. Bornscheuer,et al. Characterization and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens , 1998 .
[13] U. Bornscheuer,et al. Chemoenzymatic route to β-blockers via 3-hydroxy esters , 1996 .
[14] K. Nicolaou,et al. Chemical Biology of Epothilones. , 1998, Angewandte Chemie.
[15] Manfred T. Reetz,et al. Creation of Enantioselective Biocatalysts for Organic Chemistry by In Vitro Evolution , 1997 .
[16] S. Harayama,et al. Artificial evolution by DNA shuffling. , 1998, Trends in biotechnology.
[17] Frances H. Arnold,et al. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents , 1996, Nature Biotechnology.
[18] T. Scheper,et al. Factors affecting the lipase catalyzed transesterification reactions of 3-hydroxy esters in organic solvents. , 1993 .
[19] George M. Whitesides,et al. Enzymes in Synthetic Organic Chemistry , 1994 .