The chloroplast genome of Lappula myosotis V. Wolf, a medicinal species

Abstract Lappula myosotis V. Wolf 1776 is an annual or biennial plant with important medicinal value. In the present study, we report the complete chloroplast genome data of L. myosotis, which has a length of 146,668 bp, including a small single-copy (SSC) region of 17,059 bp, a large single-copy (LSC) region of 79,691 bp, and a pair of inverted repeats (IRs) of 24,959 bp. A total of 127 genes encoding tRNA and rRNA were annotated. The total CG content of the chloroplast genome was 37.7%. The maximum-likelihood (ML) phylogenetic tree strongly supported that L. myosotis is closely related to Trigonotis peduncularis. The complete chloroplast genome of L. myosotis provides useful information on the evolution and phylogenetic relationship among Boraginaceae plants.

[1]  J. Capelo,et al.  The complete plastome of Echium plantagineum L. (Boraginaceae), the first chloroplast genome belonging to the Echium genus , 2022, Mitochondrial DNA. Part B, Resources.

[2]  Yi He,et al.  The complete chloroplast genome of Onosma fuyunensis Y. He & Q.R. Liu and its phylogenetic analysis , 2021, Mitochondrial DNA. Part B, Resources.

[3]  Andrew D. Smith,et al.  Falco: high-speed FastQC emulation for quality control of sequencing data. , 2019, F1000Research.

[4]  Shu-Dong Zhang,et al.  The complete chloroplast genome of the multipurpose and traditional herb, Ruta graveolens L. , 2019, Mitochondrial DNA. Part B, Resources.

[5]  Patricia P. Chan,et al.  tRNAscan-SE 2.0: improved detection and functional classification of transfer RNA genes , 2019, bioRxiv.

[6]  Axel Fischer,et al.  GeSeq – versatile and accurate annotation of organelle genomes , 2017, Nucleic Acids Res..

[7]  H. Daniell,et al.  Chloroplast genomes: diversity, evolution, and applications in genetic engineering , 2016, Genome Biology.

[8]  Alexandros Stamatakis,et al.  RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies , 2014, Bioinform..

[9]  I. H. Attitalla.  Modified CTAB method for high quality genomic DNA extraction from medicinal plants. , 2011, Pakistan journal of biological sciences : PJBS.

[10]  G. Igloi,et al.  Complete sequence of the maize chloroplast genome: gene content, hotspots of divergence and fine tuning of genetic information by transcript editing. , 1995, Journal of molecular biology.

[11]  Y. Pei,et al.  [Studies on the antibacterial constituents in the fruit of Lappula echinata Gilib]. , 1986, Yao xue xue bao = Acta pharmaceutica Sinica.

[12]  Duan Hong-quan.  A new quinolone alkaloid with antibacterial activity from Lappula echinata , 2005 .