Protein disk of tobacco mosaic virus at 2.8 Å resolution showing the interactions within and between subunits

Protein subunits in the two layers of the disk of tobacco mosaic virus have very similar conformations. Much of the bonding between subunits is polar, including salt-bridge systems. Arginine residues play a prominent part here and elsewhere. Interactions within each layer involve groups whose contacts can be adjusted to allow the transition from disk to virus helix. Aromatic clusters within each molecule are linked in a hydrophobic girdle encircling each ring.

[1]  M. O. Dayhoff,et al.  Atlas of protein sequence and structure , 1965 .

[2]  A. Klug,et al.  X-ray analysis of the disk of tobacco mosaic virus protein. 3. A low resolution electron density map. , 1974, Journal of molecular biology.

[3]  P. Butler,et al.  Location of tyrosine residues in the disk of tobacco-mosaic-virus protein and comparison of the subunit packing with that of the virus. , 1978, European journal of biochemistry.

[4]  A. Klug,et al.  Rotational Symmetry of the Two Turn Disk Aggregate of Tobacco Mosaic Virus Protein , 1966, Nature.

[5]  J. Finch,et al.  Polarity of the RNA in the tobacco mosaic virus particle and the direction of protein stripping in sodium dodecyl sulphate , 1976, FEBS letters.

[6]  A. Klug,et al.  States of aggregation of tobacco mosaic virus protein. , 1971, Nature: New biology.

[7]  D. Caspar,et al.  ASSEMBLY AND STABILITY OF THE TOBACCO MOSAIC VIRUS PARTICLE. , 1963, Advances in protein chemistry.

[8]  A. Klug,et al.  Assembly of the particle of tobacco mosaic virus from RNA and disks of protein. , 1971, Nature: New biology.

[9]  G. Bricogne.  Geometric sources of redundancy in intensity data and their use for phase determination , 1974 .

[10]  D. Zimmern.  The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA , 1977, Cell.

[11]  J. Finch,et al.  Configuration of tobacco mosaic virus RNA during virus assembly , 1977, Nature.

[12]  K. Holmes,et al.  Structure of RNA and RNA binding site in tobacco mosaic virus from 4-Å map calculated from X-ray fibre diagrams , 1977, Nature.

[13]  M. Rees,et al.  The amino acid sequence of the cowpea strain of tobacco mosaic virus protein. , 1975, Biochimica et biophysica acta.

[14]  R. Huber,et al.  Structural basis of the activation and action of trypsin , 1978 .

[15]  F M Richards,et al.  The matching of physical models to three-dimensional electron-density maps: a simple optical device. , 1968, Journal of molecular biology.

[16]  K. Richards,et al.  Inside-out model for self-assembly of tobacco mosaic virus. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[17]  A. Klug,et al.  X-ray analysis of the disk of tobacco mosaic virus protein. II. The packing arrangement in the crystal. , 1974, Journal of molecular biology.

[18]  A. Jack.  Direct determination of X-ray phases for tobacco mosaic virus protein using non-crystallographic symmetry , 1973 .

[19]  T. H. Gossling,et al.  A new automatic flat-bed microdensitometer for use in x-ray crystallography. , 1977, Journal of physics E: Scientific instruments.

[20]  P. Butler,et al.  The isolation of tobacco mosaic virus RNA fragments containing the origin for viral assembly , 1977, Cell.

[21]  R. Franklin,et al.  Tobacco mosaic virus: application of the method of isomorphous replacement to the determination of the helical parameters and radial density distribution , 1958 .

[22]  G. Bricogne,et al.  Methods and programs for direct‐space exploitation of geometric redundancies , 1976 .

[23]  A Klug,et al.  Three-dimensional reconstruction of the stacked-disk aggregate of tobacco mosaic virus protein from electron micrographs. , 1971, Philosophical transactions of the Royal Society of London. Series B, Biological sciences.

[24]  A. C. Durham.  The cause of irreversible polymerisation of tobacco mosaic virus protein , 1972, FEBS letters.

[25]  O. Jardetzky,et al.  Unusual segmental flexibility in a region of tobacco mosaic virus coat protein , 1978, Nature.

[26]  G. Bricogne,et al.  The structure of the protein disk of tobacco mosaic virus to 5 Å resolution , 1976, Nature.

[27]  M. A. Lauffer.  Entropy-driven processes in biology. , 1975, Molecular biology, biochemistry, and biophysics.

[28]  T. Takano,et al.  Structure of myoglobin refined at 2-0 A resolution. I. Crystallographic refinement of metmyoglobin from sperm whale. , 1977, Journal of molecular biology.

[29]  A. Klug,et al.  X-ray analysis of the disk of tobacco mosaic virus protein. I. Crystallization of the protein and of a heavy-atom derivative. , 1974, Journal of molecular biology.