Preliminary study of fiber optic multi-cardiac-marker biosensing system for rapid coronary heart disease diagnosis and prognosis.

A fiber-optic biosensing system for the simultaneous quantification of four cardiac markers is currently under development, in our research group, for rapid coronary disease diagnosis and prognosis. As an initial effort, four individual BNP, cTnI, MG, and CRP sensors were developed. The sensors showed excellent performance in quantifying these cardiac markers in their clinically significant ranges within 15 minutes, at a S/N ratio of 25–50. The cross-reactivity of the four sensors was also found to be negligible. A mixture of four AF647-2o Mabs has shown only a slight interference to the four sensors, indicating that the mixture can be applied through a multi-sensing unit for simultaneous detection with an easier operation.