Molecular and Biochemical Evaluation of Ethyl Methanesulfonate-Induced Mutant Lines in Camelina sativa L.

Background: Camelina sativa is one of the most important oilseeds that has a proportionate profile of essential unsaturated fatty acids that are suitable for human nutrition. In this regard, we can mention a high percentage and a reasonable ratio of omega 3 and omega 6. Objectives: In the current study, the created variation of second-generation mutant (M2) camelina lines in terms of fatty acid profiles and ISSR molecular markers in C. sativa was evaluated. Materials and Methods: For this purpose, while producing the first-generation of mutant plants (M1), 200 M2 seeds with 0.1% and 0.5% ethyl methanesulfonate (EMS) mutations were treated in two replications for 8 and 16 hours based on a completely randomized design. Results: The results of mean comparisons showed that there was no significant difference between treatments in terms of fatty acids of palmitic acid, stearic acid, linoleic acid, eicosadienoic acid, oleic acid and erucic acid. The cluster analysis revealed that all the treatments used with five replications were divided into eight groups. It was found that all replications of the treatment with a concentration of 0.1% and a time of 16 hours (C1T2) were in the second group with the lowest palmitic acid was present among other treatments. Therefore, C1T2 treatment is recommended as the best treatment to reduce palmitic acid. Examination of the information content of ISSR molecular markers also showed that markers 2, 5, and 6 were the best informative markers in the detection of camelina fatty acid profiles. Conclusion: A significant variation has been created in the fatty acids profile and it can be applied in future breeding programs depending on the intended purpose.

[1]  H. Fanaei,et al.  Camelina, an adaptable oilseed crop for the warm and dried regions of Iran , 2021 .

[2]  A. Zebarjadi,et al.  Study of relationship between some agro-physiological traits with drought tolerance in rapeseed (Brassica napus L.) genotypes , 2021 .

[3]  A. Dehestani,et al.  Genetic analysis of freezing tolerance in camelina [Camelina sativa (L.) Crantz] by diallel cross of winter and spring biotypes , 2021, Planta.

[4]  M. Mostafaei,et al.  Catalytic performance of MgO /Fe2O3-SiO2 core-shell magnetic nanocatalyst for biodiesel production of Camelina sativa seed oil: Optimization by RSM-CCD method , 2021 .

[5]  M. Zarei,et al.  Characterization of physiological responses and fatty acid compositions of Camelina sativa genotypes under water deficit stress and symbiosis with Micrococcus yunnanensis , 2020 .

[6]  D. Kahrizi,et al.  Evaluation of Camelina sativa Doubled Haploid Lines for the Response to Water-deficit Stress , 2020 .

[7]  Yilin Ma,et al.  Combined Application of Arbuscular Mycorrhizal Fungi and Exogenous Melatonin Alleviates Drought Stress and Improves Plant Growth in Tobacco Seedlings , 2020, Journal of Plant Growth Regulation.

[8]  Lei Xu,et al.  An in silico approach to identification, categorization and prediction of nucleic acid binding proteins , 2020, bioRxiv.

[9]  Zhiqiang Hu,et al.  RALF1-FERONIA complex affects splicing dynamics to modulate stress responses and growth in plants , 2020, Science Advances.

[10]  A. Zebarjadi,et al.  Isolation and Characterization of Delta 15 Desaturase (FAD3) Gene From Camelina sativa L. , 2020 .

[11]  F. Fallah,et al.  Evaluation of Genetic Variation and Parameters of Fatty Acid Profile in Doubled Haploid Lines of Camelina sativa L. , 2020 .

[12]  Weiqiang Zhang,et al.  Progress of ethylene action mechanism and its application on plant type formation in crops , 2020, Saudi journal of biological sciences.

[13]  Q. Su,et al.  LncRNA TUG1 mediates ischemic myocardial injury by targeting miR-132-3p/HDAC3 axis. , 2019, American journal of physiology. Heart and circulatory physiology.

[14]  Anjiang Cai,et al.  Ordered gold nanoparticle arrays on the tip of silver wrinkled structures for single molecule detection , 2019 .

[15]  A. Zebarjadi,et al.  Endoplasmic reticulum retention signaling and transmembrane channel proteins predicted for oilseed ω3 fatty acid desaturase 3 (FAD3) genes , 2019, Functional & Integrative Genomics.

[16]  Ammar Salehisahlabadi,et al.  Socioeconomic Inequality in Fruit and Vegetable Consumptions in Elderly People: A Cross Sectional Study in North West of Iran , 2019, Nutrition and Food Sciences Research.

[17]  H. Shimelis,et al.  Seed oil content and fatty acid composition response to ethyl methanesulphonate mutagenesis in vernonia , 2019, South African Journal of Plant and Soil.

[18]  N. Altindal.  Molecular characterization of Helianthus tuberosus L. treated with ethyl methanesulfonate based on inter-simple sequence repeat markers , 2019, International Journal of Environmental Science and Technology.

[19]  Quan Zou,et al.  A Review of DNA-binding Proteins Prediction Methods , 2019, Current Bioinformatics.

[20]  Bin Liu,et al.  A Review on the Recent Developments of Sequence-based Protein Feature Extraction Methods , 2019, Current Bioinformatics.

[21]  D. Kahrizi,et al.  Effects of climate on fatty acid profile in Camelina sativa. , 2018, Cellular and molecular biology.

[22]  Fayuan Wang,et al.  Effects of arbuscular mycorrhizal inoculation and biochar amendment on maize growth, cadmium uptake and soil cadmium speciation in Cd-contaminated soil. , 2018, Chemosphere.

[23]  Chaofu Lu,et al.  Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa. , 2018, Plant physiology and biochemistry : PPB.

[24]  Y. Arslan,et al.  Molecular Characterization of Materials Selected from Some Camelina [Camelina sativa (L.) Crantz] Populations , 2017 .

[25]  Samiullah Khan,et al.  Mutagenic Effectiveness and Efficiency of Gamma Rays and HZ with Phenotyping of Induced Mutations in Lentil Cultivars , 2017, International Letters of Natural Sciences.

[26]  E. Cahoon,et al.  Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing , 2017, Plant biotechnology journal.

[27]  I. Nosratti,et al.  Molecular and agro-morphological genetic diversity assessment of Chickpea mutants induced via ethyl methane sulfonate. , 2016, Cellular and molecular biology.

[28]  D. Kahrizi,et al.  Feasibility Cultivation of Camelina (Camelina sativa) as Medicinal-Oil Plant in Rainfed Conditions in Kermanshah-Iran's First Report , 2015 .

[29]  M. I. Kozgar,et al.  Variability and Correlations Studies for Total Iron and Manganese Contents of Chickpea (Cicer arietinum L.) High Yielding Mutants , 2012 .

[30]  Tanmay Parekh,et al.  Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances , 2012, mBio.

[31]  Burton L. Johnson,et al.  Seeding date influence on camelina seed yield, yield components, and oil content in Chile. , 2011 .

[32]  E. Farshadfar,et al.  Evaluation of Genetic Diversity in Wheat Cultivars and Breeding Lines using Inter Simple Sequence Repeat Markers , 2011 .

[33]  M. M. Rad,et al.  EVALUATION OF YIELD, FATTY ACIDS COMBINATION AND CONTENT OF MICRO NUTRIENTS IN SEEDS OF HIGH YIELDING RAPESEED VARIETIES AS AFFECTED BY DIFFERENT SULPHUR RATES , 2011 .

[34]  F. Ghanati,et al.  EVALUATION OF YIELD, ITS COMPONENTS AND SOME MORPHOLOGICAL TRAITS OF SIXTEEN RAPESEED OIL CULTIVARS IN ARAK REGION , 2011 .

[35]  M. Beilstein,et al.  Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes , 2010, BMC Plant Biology.

[36]  J. Napier,et al.  Tailoring plant lipid composition: designer oilseeds come of age. , 2010, Current opinion in plant biology.

[37]  Yicun Chen,et al.  Genetic diversity and association of ISSR markers with the eleostearic content in tung tree (Vernicia fordii) , 2009 .

[38]  M. A. Rincón-Cervera,et al.  Ecological and simultaneous seed oil extraction/saponification/γ-linolenic acid concentration , 2005 .

[39]  P. Cavagnaro,et al.  Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers , 2003 .

[40]  R. Last,et al.  Ethylmethanesulfonate Saturation Mutagenesis in Arabidopsis to Determine Frequency of Herbicide Resistance , 2003, Plant Physiology.

[41]  J. Doyle,et al.  Isolation of plant DNA from fresh tissue , 1990 .

[42]  G. Lepage,et al.  Improved recovery of fatty acid through direct transesterification without prior extraction or purification. , 1984, Journal of lipid research.