Bile salts and cholesterol induce changes in the lipid cell membrane of Lactobacillus reuteri

Aims: The objective of this study was to evaluate the effect of bile salts and cholesterol in the lipid profile of Lactobacillus reuteri CRL 1098 and to determine the relationship existing between these changes: the in vitro removal of cholesterol and the tolerance of the cells to acid and cold stress.

[1]  F. Sesma,et al.  Bile salts hydrolase plays a key role on cholesterol removal by Lactobacillus reuteri , 1997, Biotechnology Letters.

[2]  F. Sesma,et al.  Localization and primary characterization of bile salt hydrolase from Lactobacillus reuteri , 1999, Biotechnology Letters.

[3]  D. de Mendoza,et al.  Mechanism of Citrate Metabolism inLactococcus lactis: Resistance against Lactate Toxicity at Low pH , 1999, Journal of bacteriology.

[4]  T. Hogg,et al.  Ethanol‐induced changes in the fatty acid composition of Lactobacillus hilgardii, its effects on plasma membrane fluidity and relationship with ethanol tolerance , 1996 .

[5]  E. Olson.  Influence of pH on bacterial gene expression , 1993, Molecular microbiology.

[6]  M. Sinensky.  Homeoviscous adaptation--a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[7]  G. Lorca,et al.  Influence of bile on beta-galactosidase activity and cell viability of Lactobacillus reuteri when subjected to freeze-drying. , 1997, Journal of dairy science.

[8]  F. Sesma,et al.  Heterofermentative pattern and exopolysaccharide production by Lactobacillus helveticus ATCC 15807 in response to environmental pH , 2001, Journal of applied microbiology.

[9]  M. Médici,et al.  Evidence for hypocholesterolemic effect of Lactobacillus reuteri in hypercholesterolemic mice. , 1998, Journal of dairy science.

[10]  M. Sanders.  Overview of Functional Foods: Emphasis on Probiotic Bacteria , 1998 .

[11]  N. Rozès,et al.  Effects of phenolic compounds on the growth and the fatty acid composition of Lactobacillus plantarum , 1998, Applied Microbiology and Biotechnology.

[12]  M. Kozempel,et al.  Changes in Membrane Fatty Acid Composition ofPediococcus sp. Strain NRRL B-2354 in Response to Growth Conditions and Its Effect on Thermal Resistance , 1999, Applied and Environmental Microbiology.

[13]  D. Bernik,et al.  Permeability and stability properties of membranes formed by lipids extracted from Lactobacillus acidophilus grown at different temperatures. , 1999, Archives of biochemistry and biophysics.

[14]  M. Hecker,et al.  Heat‐shock and general stress response in Bacillus subtilis , 1996, Molecular microbiology.

[15]  J. Boggs,et al.  Lipid intermolecular hydrogen bonding: influence on structural organization and membrane function. , 1987, Biochimica et biophysica acta.

[16]  E. Disalvo,et al.  Effect of lipid composition on the stability of cellular membranes during freeze-thawing of Lactobacillus acidophilus grown at different temperatures. , 2001, Archives of biochemistry and biophysics.

[17]  P. Aguilar,et al.  Biosynthesis and Function of Membrane Lipids , 1993 .

[18]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[19]  T. Mitsuoka.  Intestinal flora and aging. , 2009, Nutrition reviews.

[20]  H. Mykkänen,et al.  Lactobacillus reuteri as a therapeutic agent in acute diarrhea in young children. , 1997, Journal of pediatric gastroenterology and nutrition.

[21]  D. de Mendoza,et al.  Kinetics Characterization of Taurocholic Transport in Lactobacillus reuteri , 2001, Current Microbiology.