AN EXPERIMENTAL MULTI-FREQUENCY SYSTEM FOR STUDYING DOSIMETRY AND ACUTE EFFECTS ON CELL AND NUCLEAR MORPHOLOGY IN RAT TISSUES

Simultaneous exposure to multiple electromagnetic signals with widely difiering carrier frequencies is a reality of daily life, but its possible efiects on health are unknown. In this study, we exposed rats to non-thermal levels of 900 and 2450MHz TEM-mode radiation, applied individually or simultaneously, and we obtained estimates of 1g mean SAR (speciflc absorption rate) in various tissues using a numerical model of the rat and flnite-difierence time- domain software. The experimental system comprised a GTEM (gigahertz TEM) chamber connected to two vector signal generators, a signal mixer and amplifler, a directional coupler, a spectrum analyzer and a power meter. Tissue sections from rats sacriflced 24h after exposure, and from negative controls and positive controls exposed to gamma radiation, were stained with haematoxylin-eosin for evaluation of general cell morphology and with DAPI (4', 6- diamidino-2-phenylindole) for evaluation of apoptosis. Lesions, tissue destruction and apoptosis were only observed in positive controls. The results for rats exposed to either frequency, or to both simultaneously, were similar to those of unexposed negative controls. It remains to determine whether chronic exposure is similarly innocuous.

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