Toxicological Analysis of 1,4-Benzodiazepines I. Differential Pulse Polarography

A differential pulse polarographic method is described for the routine identification and quantitation of 1,4-benzodlazepines in blood or plasma with a sensitivity of 0.25-0.50 Fg/ml. The method serves as a selective screening procedure to determine the "total" concentration of a particular 1,4benzodiazepine and its metabolites prior to quantitation by more specific methods. A good correlation was shown for samples assayed by the screening method and more complicated gas chromatographic or TLC-polarographic procedures. A rapid screening procedure involving dilution of urine or gastric fluid samples and direct polarographic analysis is also detailed. In addition, a combined TLC-polarographic procedure is described to identify and quantitate the particular 1,4-benzodiazepine ingested based upon the urinary metabolic profile.

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