Formation of a Polymer Complex Between Bovine Serum Albumin and Sodium Chondroitin-6-Sulfatein an Aqueous Phase and on a Solid/Water Interface of Kaolin

Abstract A water-insoluble polyelectrolyte complex (PEC) was formed by mixing bovine serum albumin (BSA) with sodium chondroitin-6-sulfate (Na2Chs) at a pH lower than an isoelectric point (iep) of BSA. A PEC was also formed on the surface of kaolin via concurrent and/or cooperative adsorption of these two polymers on it. As a result, the aggregation of kaolin particles was induced and accelerated by virtue of an interparticle bridging effect of the PEC formed on the surface. On the other hand, water-soluble PEC was formed in an aqueous phase at a pH higher than the iep of BSA through an electrostatic attractive force between the negative charge of Na2Chs and the positive charge of patches on BSA. However, a strong effect on the aggregation of kaolin particles was not observed. The binding ratio of BSA to Na2Chs, R, in an aqueous phase and on the surface of kaolin was obtained by chemical analysis as a function of the pH of its mother solution. The R-value was affected not only by the pH but also by the in...

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