Large‐scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP‐fusion genomic DNA library

Intracellular localization is an important part of the characterization of a gene product. In an attempt to search for genes based on the intracellular localization of their products, we constructed a green fluorescent protein (GFP)‐fusion genomic DNA library of S. pombe.

[1]  M. Yanagida,et al.  The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability , 1995, The Journal of cell biology.

[2]  J. Szostak,et al.  Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombe , 1987, Molecular and cellular biology.

[3]  M. Yanagida,et al.  Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. , 1994, The EMBO journal.

[4]  D. Beach,et al.  Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules. , 1988, The EMBO journal.

[5]  T. Toda,et al.  Two novel protein kinase C‐related genes of fission yeast are essential for cell viability and implicated in cell shape control. , 1993, The EMBO journal.

[6]  S. Aves,et al.  Cloning, sequencing and transcriptional control of the Schizosaccharomyces pombe cdc10 ‘start’ gene. , 1985, The EMBO journal.

[7]  M. Yamamoto,et al.  Schizosaccharomyces pombe ste11+ encodes a transcription factor with an HMG motif that is a critical regulator of sexual development. , 1991, Genes & development.

[8]  R. Ketting,et al.  Cloning of Schizosaccharomyces pombe rph16+, a gene homologous to the Saccharomyces cerevisiae RAD16 gene. , 1996, Mutation research.

[9]  J. Thorner,et al.  A novel FK506- and rapamycin-binding protein (FPR3 gene product) in the yeast Saccharomyces cerevisiae is a proline rotamase localized to the nucleolus , 1994, The Journal of cell biology.

[10]  A. Goffeau,et al.  Yeast genome , 1995 .

[11]  M. Sekiguchi Genes to cells: edited by Jun-ichi Tomizawa, Blackwell Science Ltd. Institutional: £218.00 (Europe), £242.00 (Rest of World), US$382.00 (USA and Canada). Individual: £65.00 (Europe), £72.00 (Rest of World), US$114.00 (USA and Canada) ISSN 1356 9597 , 1997 .

[12]  K. Maundrell Thiamine-repressible expression vectors pREP and pRIP for fission yeast. , 1993, Gene.

[13]  Y. Hiraoka,et al.  Oscillatory nuclear movement in fission yeast meiotic prophase is driven by astral microtubules, as revealed by continuous observation of chromosomes and microtubules in living cells. , 1998, Journal of cell science.

[14]  T. Mélèse,et al.  Yeast NPI46 encodes a novel prolyl cis-trans isomerase that is located in the nucleolus , 1994, The Journal of cell biology.

[15]  D. Bouvier,et al.  Characterization of the POL3 gene product from Schizosaccharomyces pombe indicates inter-species conservation of the catalytic subunit of DNA polymerase delta. , 1991, Journal of molecular biology.

[16]  T. Toda,et al.  A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast. , 1995, The EMBO journal.

[17]  J. Potashkin,et al.  U2AF homolog required for splicing in vivo. , 1993, Science.

[18]  P. Nurse,et al.  Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[19]  T. Toda,et al.  A Novel Nuclear Export Signal Sensitive to Oxidative Stress in the Fission Yeast Transcription Factor Pap1* , 1999, The Journal of Biological Chemistry.

[20]  J. Bähler,et al.  Unusual nuclear structures in meiotic prophase of fission yeast: a cytological analysis , 1993, The Journal of cell biology.

[21]  S. Moreno,et al.  Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. , 1991, Methods in enzymology.

[22]  A. Reymond,et al.  The S. pombe cdc15 gene is a key element in the reorganization of F-actin at mitosis , 1995, Cell.

[23]  Hans-Werner Mewes,et al.  the yeast genome , 1997 .

[24]  Y. Hiraoka,et al.  Dynamics of chromosomes and microtubules visualized by multiple‐wavelength fluorescence imaging in living mammalian cells: effects of mitotic inhibitors on cell cycle progression , 1997, Genes to cells : devoted to molecular & cellular mechanisms.

[25]  M. Rout,et al.  POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope , 1994, The Journal of cell biology.

[26]  A. Dominguez,et al.  Analysis of the ntp1+ gene, encoding neutral trehalase in the fission yeast Schizosaccharomyces pombe. , 1998, Biochimica et biophysica acta.

[27]  K. Dimitrov,et al.  The Role of fnx1, a Fission Yeast Multidrug Resistance Protein, in the Transition of Cells to a Quiescent G0 State , 1998, Molecular and Cellular Biology.

[28]  O. Niwa,et al.  A Novel Fission Yeast Gene, tht1 +, Is Required for the Fusion of Nuclear Envelopes during Karyogamy , 1998, The Journal of cell biology.

[29]  M. Yanagida,et al.  Mutations in fission yeast Cut15, an importin α homolog, lead to mitotic progression without chromosome condensation , 1998, Current Biology.

[30]  B. Wilkinson,et al.  Cloning of SEC61 homologues from Schizosaccharomyces pombe and Yarrowia lipolytica reveals the extent of functional conservation within this core component of the ER translocation machinery. , 1997, Journal of cell science.

[31]  R. Laskey,et al.  Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: Identification of a class of bipartite nuclear targeting sequence , 1991, Cell.

[32]  R. Laskey,et al.  Nuclear targeting sequences--a consensus? , 1991, Trends in biochemical sciences.

[33]  K. Hoe,et al.  Isolation of a novel heat shock protein 70-like gene, pss1+ of Schizosaccharomyces pombe homologous to hsp110/SSE subfamily. , 1998, Gene.

[34]  Masayuki Yamamoto,et al.  Identification of Myo3, a second type‐II myosin heavy chain in the fission yeast Schizosaccharomyces pombe , 1997, FEBS letters.

[35]  K. Nakai,et al.  PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. , 1999, Trends in biochemical sciences.

[36]  Paul Russell,et al.  Negative regulation of mitosis by wee1 +, a gene encoding a protein kinase homolog , 1987, Cell.

[37]  J. Chernoff,et al.  The fission yeast genes pyp1+ and pyp2+ encode protein tyrosine phosphatases that negatively regulate mitosis , 1992, Molecular and cellular biology.

[38]  P. Russell,et al.  Negative regulation of mitosis by two functionally overlapping PTPases in fission yeast. , 1992, The EMBO journal.

[39]  T. Enoch,et al.  Suppressors of cdc25p overexpression identify two pathways that influence the G2/M checkpoint in fission yeast. , 1998, Genetics.

[40]  B. Lapeyre,et al.  Study of multiple fibrillarin mRNAs reveals that 3' end formation in Schizosaccharomyces pombe is sensitive to cold shock. , 1993, Nucleic acids research.

[41]  André Goffeau,et al.  The yeast genome directory. , 1997, Nature.

[42]  M. Yanagida,et al.  Telomere-led premeiotic chromosome movement in fission yeast. , 1994, Science.

[43]  I. Rupeš,et al.  Ssp1 promotes actin depolymerization and is involved in stress response and new end take-off control in fission yeast. , 1999, Molecular biology of the cell.

[44]  M. Yamamoto,et al.  Schizosaccharomyces pombe sxa1+ and sxa2+ encode putative proteases involved in the mating response , 1992, Molecular and cellular biology.

[45]  J. Hayles,et al.  Cloning and sequencing of the cyclin-related cdc13+ gene and a cytological study of its role in fission yeast mitosis. , 1988, Journal of cell science.

[46]  G. Cottarel Mcs4, a two-component system response regulator homologue, regulates the Schizosaccharomyces pombe cell cycle control. , 1997, Genetics.

[47]  J. Swedlow,et al.  Three-dimensional multiple-wavelength fluorescence microscopy for the structural analysis of biological phenomena. , 1991, Seminars in cell biology.

[48]  Stephen S. Taylor,et al.  A Visual Screen of a Gfp-Fusion Library Identifies a New Type of Nuclear Envelope Membrane Protein , 1999, The Journal of cell biology.

[49]  T. Toda,et al.  Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases. , 1991, Genes & development.

[50]  Nihon Hassei Seibutsu Gakkai,et al.  Genes to cells , 1996 .

[51]  C DeLisi,et al.  The detection and classification of membrane-spanning proteins. , 1985, Biochimica et biophysica acta.

[52]  M. Kanehisa,et al.  A knowledge base for predicting protein localization sites in eukaryotic cells , 1992, Genomics.

[53]  A. Casamayor,et al.  Regulation of salt tolerance in fission yeast by a protein-phosphatase-Z-like Ser/Thr protein phosphatase. , 1997, European journal of biochemistry.

[54]  C. Cantor,et al.  Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome. , 1989, Nucleic acids research.

[55]  G. Fink,et al.  NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex. , 1993, Molecular biology of the cell.

[56]  Walter Keller,et al.  The Schizosaccharomyces pombe pla1 gene encodes a poly(A) polymerase and can functionally replace its Saccharomyces cerevisiae homologue , 1996, Nucleic Acids Res..

[57]  N. Raikhel,et al.  Protein import into the nucleus: an integrated view. , 1995, Annual review of cell and developmental biology.

[58]  R. Kingston,et al.  Heat shock factor is required for growth at normal temperatures in the fission yeast Schizosaccharomyces pombe , 1993, Molecular and cellular biology.

[59]  R. Wepf,et al.  A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution. , 1994, The EMBO journal.

[60]  T. Pollard,et al.  Identification of a second myosin-II in Schizosaccharomyces pombe: Myp2p is conditionally required for cytokinesis. , 1997, Molecular biology of the cell.

[61]  J. S. Miles,et al.  Structurally and functionally conserved regions of cytochrome P-450 reductase as targets for DNA amplification by the polymerase chain reaction. Cloning and nucleotide sequence of the Schizosaccharomyces pombe cDNA. , 1992, The Biochemical journal.

[62]  S. Kim,et al.  Isolation and characterization of hrp1+, a new member of the SNF2/SWI2 gene family from the fission yeast Schizosaccharomyces pombe , 1998, Molecular and General Genetics MGG.

[63]  K. Sakka,et al.  Cloning and characterization of two genes encoding dihydroxyacetone kinase from Schizosaccharomyces pombe IFO 0354. , 1998, Biochimica et biophysica acta.

[64]  J. Heitman,et al.  Nuclear protein localization. , 1991, Biochimica et biophysica acta.

[65]  Fred Chang,et al.  cdc12p, a Protein Required for Cytokinesis in Fission Yeast, Is a Component of the Cell Division Ring and Interacts with Profilin , 1997, The Journal of cell biology.

[66]  J. Carbon,et al.  Isolation and characterization of the structural gene for secreted acid phosphatase from Schizosaccharomyces pombe. , 1986, The Journal of biological chemistry.

[67]  P. Ross-Macdonald,et al.  Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. , 1994, Genes & development.

[68]  A. Pidoux,et al.  Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function. , 1996, Molecular biology of the cell.

[69]  R. Tsien,et al.  Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer , 1996, Current Biology.

[70]  S. Sazer,et al.  Characterization of a Nuclear Protein Conferring Brefeldin A Resistance in Schizosaccharomyces pombe(*) , 1996, The Journal of Biological Chemistry.