APPRAISAL OF FIVE DIFFERENT PHENOTYPIC EDIBLE BIVALVES CRUDE PROTEIN EXTRACT FOR MICROBICIDAL POTENCY

The stretches of microbial infections are abiding from ancient days to till date with different symptoms and severity. Most of the modern medicines were failed to treat the infection without side effects. The resistance developed by the microbial strains is also harmfully increased. To overcome all these effects the research was focused upon bioactive natural products from various types of natural sources. Based on this idea we work towards identification of antimicrobial proteins from an edible bivalve (Donax cuneatus). Fresh bivalves of five different phenotypic characters were collected from the coast of Cuddalore. The bivalves were named as P1, P2, P3, P4 and P5. The fleshes of the bivalves were then removed and the crude protein was extracted from each type with 5% of cold acetic acid in water solvent, centrifuged at 4˚C and partially purified by ammonium sulfate precipitation. The precipitate was stored at -20˚C still evaluation. The precipitate was diluted in double distilled water and antimicrobial activity was assayed by well diffusion method against few strains of bacterial clinical isolates. All the extracts were potential against the tested microbial strains. The maximum zone of 26mm, 25mm and 20mm was observed with P5 extract against Bacillus sp, Staphylococcus sp and Proteus sp respectively and a minimum of 8mm zone was observed with P2 extract against Pseudomonas sp, Salmonella sp, Shigella sp. 1mg of protein in 1ml of P5 precipitate was estimated by Lowry's method. All the other types had the protein concentration not less than 0.9mg/ml. The SDS and FTIR studies also portrayed the presence of proteins in the crude extract of P5.