Bicompartmental device for dynamic cell coculture: design, realisation and preliminary results.

Low-shear perfusion and compartmental systems are very important conceptual designs for the realization of high-density cell cultures and cocultures, thus allowing very realistic models and therefore reliable results. In this work, we combines low-shear perfusion and multi-compartments in one device. We also describe the design, fabrication and validation of a bicompartmental, low-perfusion culture chamber specifically designed for applications in various fields, such as tissue engineering, pharmacology, molecular biology, pharmacokinetics, nanomedicine, and drug delivery testing. The device properties in terms of flow features and metabolite exchange capabilities are fully characterized. In vitro tests performed on cell lines confirm the effectiveness of the device as an experimental tool for measurements in dynamic and multiple-cell cultures and cocultures.

[1]  Michael L Shuler,et al.  Incorporation of 3T3‐L1 Cells To Mimic Bioaccumulation in a Microscale Cell Culture Analog Device for Toxicity Studies , 2008, Biotechnology progress.

[2]  P. Dario,et al.  Magnetic Driven Alginate Nanoparticles for Targeted Drug Delivery , 2008 .

[3]  L M Sweeney,et al.  A cell culture analogue of rodent physiology: Application to naphthalene toxicology. , 1995, Toxicology in vitro : an international journal published in association with BIBRA.

[4]  Ralf Pörtner,et al.  Perfusion cultures and modelling of oxygen uptake with three-dimensional chondrocyte pellets , 1999 .

[5]  P. Pawson,et al.  Physiological differences between strong and weak frog neuromuscular junctions: a study involving tetanic and posttetanic potentiation , 1990, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[6]  R M Nerem,et al.  The elongation and orientation of cultured endothelial cells in response to shear stress. , 1985, Journal of biomechanical engineering.

[7]  G. Whitesides,et al.  Complex Optical Surfaces Formed by Replica Molding Against Elastomeric Masters , 1996, Science.

[8]  D. A. Brown,et al.  Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice , 1990, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[9]  E. Orwin,et al.  Bioreactor design for cornea tissue engineering: Material-cell interactions. , 2007, Acta biomaterialia.

[10]  B. Berk,et al.  Laminar shear stress: mechanisms by which endothelial cells transduce an atheroprotective force. , 1998, Arteriosclerosis, thrombosis, and vascular biology.

[11]  C. Knowles,et al.  Mammalian cell damage in a novel membrane bioreactor , 1994, Biotechnology and bioengineering.

[12]  C. Stoscheck,et al.  Quantitation of protein. , 1990, Methods in enzymology.

[13]  Ralf Pörtner,et al.  Long-term cultivation of immortalised mouse hepatocytes in a high cell density, fixed-bed reactor , 1998 .

[14]  Ralf Pörtner,et al.  Bioreactor design for tissue engineering. , 2005, Journal of bioscience and bioengineering.

[15]  G. Whitesides,et al.  Soft Lithography. , 1998, Angewandte Chemie.

[16]  Teruo Fujii,et al.  Cell Culture in 3-Dimensional Microfluidic Structure of PDMS (polydimethylsiloxane) , 2003 .

[17]  P. Dario,et al.  Alginate and chitosan particles as drug delivery system for cell therapy , 2008, Biomedical microdevices.

[18]  A. Zeiher,et al.  Shear Stress–Induced Endothelial Cell Migration Involves Integrin Signaling Via the Fibronectin Receptor Subunits α5 and β1 , 2002 .

[19]  P. Dario,et al.  Design criteria of neuron/electrode interface. The focused ion beam technology as an analytical method to investigate the effect of electrode surface morphology on neurocompatibility , 2007, Biomedical microdevices.

[20]  D. Wendt,et al.  The role of bioreactors in tissue engineering. , 2004, Trends in biotechnology.

[21]  J. Brugge,et al.  Integrins and signal transduction pathways: the road taken. , 1995, Science.

[22]  M. Ferrari.  Nanovector therapeutics. , 2005, Current opinion in chemical biology.

[23]  D. Hayoz,et al.  Influence of oscillatory and unidirectional flow environments on the expression of endothelin and nitric oxide synthase in cultured endothelial cells. , 1998, Arteriosclerosis, thrombosis, and vascular biology.

[24]  A. Ahluwalia,et al.  Controlled in vitro growth of cell microtubes: towards the realisation of artificial microvessels , 2008, Biomedical microdevices.

[25]  M. Shuler,et al.  Bioreactor for the study of defined interactions of toxic metals and biofilms , 1985, Applied and environmental microbiology.

[26]  Teruo Fujii,et al.  Perfusion culture of fetal human hepatocytes in microfluidic environments , 2004 .

[27]  Miroslav Zivkovic,et al.  A finite element model of cell deformation during magnetic bead twisting. , 2002, Journal of applied physiology.

[28]  Feng Zhao,et al.  Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: dynamic cell seeding and construct development. , 2005, Biotechnology and bioengineering.

[29]  James H. Brown,et al.  A General Model for the Origin of Allometric Scaling Laws in Biology , 1997, Science.

[30]  Jyeshtharaj B. Joshi,et al.  Turbulent shear stress—Effect on mammalian cell culture and measurement using laser Doppler anemometer , 1995 .

[31]  D. Yee,et al.  Characterization of an in vitro cell culture bioreactor system to evaluate anti-neoplastic drug regimens , 2006, Breast Cancer Research and Treatment.