THE RELATIONSHIP BETWEEN FIXATION AND TECHNIQUES FOR THE HISTOCHEMICAL LOCALIZATION OF HYDROLYTIC ENZYMES

For (‘nzyuiie locahizations, thuere exists a (‘lose interrelatiouishih) between initial fixation , substrate use(l, products of hydrolysis an(l the final dyestuff pattern. In a general way an enzyme techiniquue is no better than the state of fixation of a giveti tissue. For example, ati azo-dye procedure design(’d to give lrecise histochemical locahizatHins may have limited use with acetone-fixe(l

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