An Optical Coherence Microscope for 3-dimensional Imaging in Developmental Biology References and Links

An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.

[1]  Adolf Friedrich Fercher.  Optical coherence tomography. , 1996 .

[2]  B E Bouma,et al.  Rapid acquisition of in vivo biological images by use of optical coherence tomography. , 1996, Optics letters.

[3]  A Rollins,et al.  In vivo video rate optical coherence tomography. , 1998, Optics express.

[4]  B E Bouma,et al.  Two- and three-dimensional high-resolution imaging of the human oviduct with optical coherence tomography. , 1998, Fertility and sterility.

[5]  J. Fujimoto,et al.  Intraoperative assessment of microsurgery with three-dimensional optical coherence tomography. , 1998, Radiology.

[6]  J. Fujimoto,et al.  Noninvasive assessment of the developing Xenopus cardiovascular system using optical coherence tomography. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[7]  S A Boppart,et al.  Optical coherence tomography for neurosurgical imaging of human intracortical melanoma. , 1998, Neurosurgery.

[8]  J. Izatt,et al.  Optical coherence microscopy in gastrointestinal tissues , 1996, Summaries of papers presented at the Conference on Lasers and Electro-Optics.

[9]  B R Masters,et al.  Early development of optical low-coherence reflectometry and some recent biomedical applications. , 1999, Journal of biomedical optics.

[10]  M. Bashkansky,et al.  Subsurface defect detection in materials using optical coherence tomography. , 1998, Optics express.

[11]  T. Yano,et al.  Piezoelectrically induced optical phase modulation of light in single-mode fibers , 1992 .

[12]  Stephen A. Boppart,et al.  Imaging developing neural morphology using optical coherence tomography , 1996, Journal of Neuroscience Methods.

[13]  Brett E. Bouma,et al.  In vivo cellular optical coherence tomography imaging , 1998, Nature Medicine.

[14]  Eric A. Swanson,et al.  Blindness limitations in optical coherence domain reflectometry , 1993 .

[15]  J. Izatt,et al.  High resolution imaging of in vivo cardiac dynamics using color Doppler optical coherence tomography. , 1997, Optics express.

[16]  J. Fujimoto,et al.  Investigation of developing embryonic morphology using optical coherence tomography. , 1996, Developmental biology.

[17]  J. Izatt,et al.  Optical Coherence Tomography and Microscopy in Gastrointestinal Tissues , 1996, Advances in Optical Imaging and Photon Migration.

[18]  W. Sorin,et al.  A simple intensity noise reduction technique for optical low-coherence reflectometry , 1992, IEEE Photonics Technology Letters.