Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates.

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) for identification of Bcc species compared with that of recA sequencing. MALDI-ToF was able of identifying 100% of Bcc isolates at the genus level, but 23.1% of Bcc isolates tested were not correctly identified at the species level. The misidentification occurred most frequently with Burkholderia contaminans (100%) and B. cepacia (33.3%).

[1]  Prasanna D. Khot,et al.  Optimization of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis for Bacterial Identification , 2012, Journal of Clinical Microbiology.

[2]  L. Torbeck,et al.  Burkholderia cepacia: This Decision Is Overdue , 2011, PDA Journal of Pharmaceutical Science and Technology.

[3]  S. Richter,et al.  Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cultures from Cystic Fibrosis Patients , 2012, Journal of Clinical Microbiology.

[4]  J. Lipuma.  The Changing Microbial Epidemiology in Cystic Fibrosis , 2010, Clinical Microbiology Reviews.

[5]  V. Raia,et al.  Rapid identification of Burkholderia cepacia complex species recovered from cystic fibrosis patients using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. , 2013, Journal of microbiological methods.

[6]  Delphine Martiny,et al.  Comparison of the Microflex LT and Vitek MS Systems for Routine Identification of Bacteria by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry , 2012, Journal of Clinical Microbiology.

[7]  M. Hogardt,et al.  Hospital-wide outbreak of Burkholderia contaminans caused by prefabricated moist washcloths. , 2011, The Journal of hospital infection.

[8]  P. Woo,et al.  Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Burkholderia pseudomallei: Importance of Expanding Databases with Pathogens Endemic to Different Localities , 2012, Journal of Clinical Microbiology.

[9]  A. Bizzini,et al.  Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. , 2010, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[10]  C. Dowson,et al.  Burkholderia cepacia complex bacteria: opportunistic pathogens with important natural biology , 2008, Journal of applied microbiology.

[11]  M. Maley,et al.  Utility of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry following Introduction for Routine Laboratory Bacterial Identification , 2011, Journal of Clinical Microbiology.

[12]  J. Lipuma,et al.  Use of matrix assisted laser desorption ionisation–time of flight mass spectrometry in a paediatric clinical laboratory for identification of bacteria commonly isolated from cystic fibrosis patients , 2012, Journal of Clinical Pathology.

[13]  R. Cantón,et al.  MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients. , 2012, Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society.

[14]  C. Vay,et al.  Genetic Diversity of Burkholderia contaminans Isolates from Cystic Fibrosis Patients in Argentina , 2012, Journal of Clinical Microbiology.

[15]  P. Berche,et al.  Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Nonfermenting Gram-Negative Bacilli Isolated from Cystic Fibrosis Patients , 2008, Journal of Clinical Microbiology.

[16]  R. Kessler,et al.  Should cystic fibrosis patients infected with Burkholderia cepacia complex be listed for lung transplantation? , 2011, Interactive cardiovascular and thoracic surgery.

[17]  A. Mellmann,et al.  Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting Bacteria , 2008, Journal of Clinical Microbiology.

[18]  G Greub,et al.  Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Bacterial Strains Routinely Isolated in a Clinical Microbiology Laboratory , 2010, Journal of Clinical Microbiology.