Effect of hyperthermia on glutathione peroxidase and lipid peroxidative damage in liver

1. 1.|Significant lipid peroxidation occurred both in rat and guinea pig liver in passive hyperthermia caused by a hot environment. In guinea pig, lipid peroxidation was greatly induced not only in liver homogenate but also in intracellular structures, such as mitochondria and microsomes. 2. 2.|In rat, hepatic cytosolic selenium glutathione peroxidase activities were greatly induced in hyperthermia, while hepatic cytosolic glutathione peroxidase activities in guinea pig were not affected in hyperthermia. 3. 3.|In both animals, hepatic cytosolic glutathione S-transferase, cytosolic superoxide dismutase, and catalase activities were not affected. 4. 4.|Since the peroxidation of lipids in biological membranes is a destructive phenomenon and is associated with a variety of cellular damage, hyperthermia greatly developed hypertrophy and vacuolized degeneration in hepatic cells. 5. 5.|In guinea pig, the activities of hepatic mitochondrial electron transport system, such as cytochrome c oxidase and cytochrome c reductase system were simultaneously inhibited in hyperthermia. 6. 6.|On the other hand, hepatic microsomal electron transport system, such as cytochrome P450 and aminopyrine N-demethylase activities were not seriously affected by hyperthermia. 7. 7.|In nuclei and mitochondria, 90-kDa heat shock induced proteins markedly expressed in liver. 8. 8.|Since hyperthermia greatly induces lipid peroxidation in liver, heat stress may have a potential impact on peroxidative damage in humans and animals.

[1]  M. Noshiro,et al.  Immunochemical study on the electron pathway from NADH to cytochrome P-450 of liver microsomes. , 1978, Journal of biochemistry.

[2]  K. Yagi,et al.  Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. , 1979, Analytical biochemistry.

[3]  Critical upper levels of body temperature, tissue thermosensitivity and selective brain cooling in hyperthermia. , 1987 .

[4]  R. Burk,et al.  Glutathione peroxidase activity in selenium-deficient rat liver. , 1976, Biochemical and biophysical research communications.

[5]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[6]  R. O. Recknagel,et al.  Destruction of microsomal cytochrome P-450 and glucose-6-phosphatase by lipids extracted from peroxidized microsomes. , 1978, Toxicology and applied pharmacology.

[7]  M. Oppenheimer,et al.  Developing policies for responding to climatic change , 1989 .

[8]  J. Werringloer.  Assay of formaldehyde generated during microsomal oxidation reactions. , 1978, Methods in enzymology.

[9]  W. Pryor,et al.  The inhibitory effect of extracts of cigarette tar on electron transport of mitochondria and submitochondrial particles. , 1992, Free radical biology & medicine.

[10]  M. Marmor.  Heat wave mortality in nursing homes. , 1978, Environmental research.

[11]  L. Oberley,et al.  Superoxide dismutase levels in Chinese hamster ovary cells and ovarian carcinoma cells after hyperthermia or exposure to cycloheximide. , 1985, Cancer research.

[12]  W. Tegart,et al.  Climate change : the IPCC impacts assessment , 1990 .

[13]  F. Tietze.  Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. , 1969, Analytical biochemistry.

[14]  Seth Sadis,et al.  Secondary structure of the mammalian 70-kilodalton heat shock cognate protein analyzed by circular dichroism spectroscopy and secondary structure prediction. , 1990, Biochemistry.

[15]  R. Burk,et al.  Non-Selenium-Dependent Glutathione Peroxidase , 1978 .

[16]  W. Valentine,et al.  Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. , 1967, The Journal of laboratory and clinical medicine.

[17]  F. Hartl,et al.  Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis , 1989, Nature.

[18]  R. Burdon,et al.  Heat shock and the heat shock proteins. , 1986, The Biochemical journal.

[19]  F. Cajone,et al.  4-Hydroxynonenal induces a DNA-binding protein similar to the heat-shock factor. , 1989, The Biochemical journal.

[20]  D. Miller,et al.  Xanthine oxidase- and iron-dependent lipid peroxidation. , 1993, Archives of biochemistry and biophysics.

[21]  M. Welsh,et al.  Interaction of the glucocorticoid receptor with the Mr 90,000 heat shock protein: an evolving model of ligand-mediated receptor transformation and translocation. , 1989, Cancer research.

[22]  S. Orrenius.  ON THE MECHANISM OF DRUG HYDROXYLATION IN RAT LIVER MICROSOMES , 1965, The Journal of cell biology.

[23]  R. Burk,et al.  Species, tissue and subcellular distribution of non Se-dependent glutathione peroxidase activity. , 1978, The Journal of nutrition.

[24]  W. Pryor,et al.  tert-Butyl hydroperoxide-induced radical production in rat liver mitochondria. , 1992, Free radical biology & medicine.

[25]  John Bligh.  Heat stress: Physical exertion and environment: J. R. S. Hales and D. A. B. Richards (Eds), 525 pp. Excerpta Medical, Amsterdam, 1987 , 1989 .

[26]  S. Colowick,et al.  Methods in Enzymology , Vol , 1966 .

[27]  T. Omura,et al.  THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE. , 1964, The Journal of biological chemistry.

[28]  W. Keatinge,et al.  Increased platelet and red cell counts, blood viscosity, and plasma cholesterol levels during heat stress, and mortality from coronary and cerebral thrombosis. , 1986, The American journal of medicine.

[29]  U. K. Laemmli,et al.  Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 , 1970, Nature.

[30]  T. Slater,et al.  Studies on the reduction of nitroblue tetrazolium chloride mediated through the action of NADH and phenazine methosulphate. , 1978, Chemico-biological interactions.

[31]  T. Mantle,et al.  Glutathione S-transferases. , 1990, Biochemical Society transactions.

[32]  S B Thacker,et al.  Morbidity and mortality associated with the July 1980 heat wave in St Louis and Kansas City, Mo. , 1982, JAMA.

[33]  H. Aebi,et al.  Catalase in vitro. , 1984, Methods in enzymology.

[34]  D. Tirpak,et al.  The Potential Effects of Global Climate Change on the United States , 1990 .

[35]  E. Rogot,et al.  Associations of coronary and stroke mortality with temperature and snowfall in selected areas of the United States, 1962-1966. , 1976, American journal of epidemiology.

[36]  A. Tappel,et al.  Methyl ethyl ketone peroxide damage to cytochrome P-450 peroxidase activities. , 1985, Toxicology and applied pharmacology.

[37]  A. Astromoff,et al.  Cellular glutathione, thermal sensitivity, and thermotolerance in Chinese hamster fibroblasts and their heat-resistant variants. , 1986, Cancer research.

[38]  Ethyl Ketone.  Methyl ethyl ketone. , 1988, Reviews of environmental contamination and toxicology.

[39]  L. Kalkstein,et al.  Weather and Human Mortality: An Evaluation of Demographic and Interregional Responses in the United States , 1989 .

[40]  W B Jakoby,et al.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. , 1974, The Journal of biological chemistry.