Light and electron microscopy studies of the infection of the western flower thrips frankliniella occidentalis (Thysanoptera: thripidae) by the entomopathogenic fungus metarhizium anisopliae

The infection of Frankliniella occidentalis by two isolates of the entomopathogenic fungus Metarhizium anisopliae was studied using fluorescence, scanning, transmission, and confocal scanning laser microscopy techniques. Conidia of M. anisopliae adhered mostly to the wings of adult F. occidentalis but the number declined from 73 to 40% within 72 h postinoculation at 23 degreesC, presumably due to preening. Conidia germinated and produced appressoria on adult, larval, and pupal stages within 12 h post inoculation. However, penetration pegs were not observed until 30 h postinoculation. Light microscopy and transmission electron microscopy provided further information on fungal development inside the insect. The fungus colonized the insect hemocoel from day 3 and sporulated approximately 6 days postinoculation. Copyright 1999 Academic Press.

[1]  D. D. Perkins.  PRESERVATION OF NEUROSPORA STOCK CULTURES WITH ANHYDROUS SILICA GEL , 1962 .

[2]  E. Reynolds.  THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY , 1963, The Journal of cell biology.

[3]  R. Zacharuk.  Fine structure of the fungus Metarrhizium anisopliae infecting three species of larval Elateridae (Coleoptera): III. Penetration of the host integument , 1970 .

[4]  R. Hall.  A bioassay of the pathogenicity of Verticillium lecanii conidiospores on the aphid, Macrosiphoniella sanborni , 1976 .

[5]  J. Fargues,et al.  Histological and ultrastructural studies of Beauveria bassiana infection in Leptinotarsa decemlineta larvae during ecdysis , 1977 .

[6]  H. Schabel.  Percutaneous infection of Hylobius pales by Metarrhizium anisopliae , 1978 .

[7]  Alan Beckett,et al.  Low-Temperature Scanning Electron Microscopy , 1986 .

[8]  L. Yudin,et al.  Host Range of Western Flower Thrips, Frankliniella occidentalis (Thysanoptera: Thripidae), with Special Reference to Leucaena glauca , 1986 .

[9]  W. Todd,et al.  Ultrastructure Techniques for Microorganisms , 1986, Springer US.

[10]  R. Davies.  Outlines of Entomology , 1988, Springer Netherlands.

[11]  S. Gunnarsson.  Infection of Schistocerca gregaria by the fungus Metarhizium anisopliae: Cellular reactions in the integument studied by scanning electron and light microscopy , 1988 .

[12]  R. Leger,et al.  Role of extracellular chymoelastase in the virulence of Metarhizium anisopliae for Manduca sexta , 1988 .

[13]  R. Staples,et al.  Use of fluorochromes in the study of fungal cytology and differentiation , 1989 .

[14]  L. Allee,et al.  Germination and infection processes of the entomophthoralean fungus Erynia radicans on the potato leafhopper, Empoasca fabae , 1990 .

[15]  A. Charnley.  Secondary metabolites, toxins and entomopathogenic fungi: an evolutionary perspective. , 1990 .

[16]  W. Ravensberg,et al.  Verticillium lecanii as a microbial insecticide against glasshouse whitefly. , 1990 .

[17]  K. Binnington,et al.  Physiology of the insect epidermis , 1991 .

[18]  D. Boucias,et al.  Attachment of Mycopathogens to Cuticle , 1991 .

[19]  R. Staples,et al.  Prepenetration events during infection of host cuticle by , 1991 .

[20]  H. C. Hoch,et al.  The Fungal Spore and Disease Initiation in Plants and Animals , 1991, Springer US.

[21]  R. Humber,et al.  Biotechnology of Fungi for Improving Plant Growth , 1992 .

[22]  A. Beckett,et al.  Germination and the role of extracellular matrix in adhesion of urediniospores of Uromyces viciae-fabae to synthetic surfaces , 1993 .

[23]  Anton Hartmann,et al.  Direct identification, and localization of Azospirillum in the rhizosphere of wheat using fluorescence‐labelled monoclonal antibodies and confocal scanning laser microscopy , 1993 .

[24]  R. S. St. Leger,et al.  INTERACTIONS BETWEEN FUNGAL PATHOGENS AND INSECT HOSTS , 1994 .

[25]  David W Williams,et al.  Investigation of the value of β-glucan-specific fluorochromes for predicting the β-glucan content of the cell walls of zoopathogenic fungi , 1994 .

[26]  A. Beckett,et al.  Invasion and development of Verticillium lecanii in the western flower thrips, Frankliniella occidentalis , 1994 .

[27]  J. Eilenberg,et al.  Pathogenicity of the Hyphomycete Fungi Verticillium lecanii and Metarhizium anisopliae to the Western Flower Thrips, Frankliniella occidentalis , 1995 .

[28]  Alan Beckett,et al.  The germination behaviour of Metarhizium anisopliae on the surface of aphid and flea beetle cuticles , 1995 .

[29]  T. Butt.  Chapter VIII-2 – Complementary techniques: Fluorescence microscopy , 1997 .

[30]  L. Lacey.  Manual of techniques in insect pathology , 1997 .