Simultaneous Inoculation of Malbec (Vitis vinifera) Musts with Yeast and Bacteria: Effects on Fermentation Performance, Sensory and Sanitary Attributes of Wines

Summary Malolactic fermentation has important consequences for the quality of wine. The present study analyzes the effect of timing of inoculation with bacteria on the performance of alcoholic and malolactic fermentation in Malbec musts from a warm climate wine growing region during two consecutive vintages, with a specific consideration for the sensory and sanitary wine parameters. In this study traditional vinification, where malolactic bacteria were inoculated after the completion of alcoholic fermentation, was compared with a simultaneous inoculation with yeast and bacteria. The experiment was made on pilot scale which closely reproduces winery conditions. The obtained results point out that simultaneous inoculation resulted in a reduction of total time of fermentation and a better control of the malolactic fermentation due to the early dominance of a selected bacterial strain. There were no negative effects on yeast population and alcoholic fermentation performance observed. Differences between the wine sensory attributes were no significant or they were in favour of simultaneous inoculations. No statistical differences in the biogenic amine levels between different timings of inoculation were found.

[1]  J. M. Landete,et al.  Biogenic amine production by lactic acid bacteria, acetic bacteria and yeast isolated from wine , 2007 .

[2]  S. Krieger-Weber,et al.  Simultaneous and sequential alcoholic and malolitic fermentations: a comparison for Amarone-style wines , 2007 .

[3]  G. Zapparoli,et al.  Comparison between simultaneous and sequential alcoholic and malolactic fermentations for partially dried grapes in the production of Amarone style wine , 2007 .

[4]  R. Mira de Orduña,et al.  Effect of Simultaneous Inoculation with Yeast and Bacteria on Fermentation Kinetics and Key Wine Parameters of Cool-Climate Chardonnay , 2006, Applied and Environmental Microbiology.

[5]  G. Zapparoli,et al.  Influence of the pH of Chardonnay must on malolactic fermentation induced by bacteria co-inoculated with yeasts , 2006 .

[6]  Kathleen J. Arnink,et al.  Influence of Saccharomyces cerevisiae and Oenococcus oeni Strains on Successful Malolactic Conversion in Wine , 2005, American Journal of Enology and Viticulture.

[7]  F. Clementi,et al.  Interactions between Saccharomyces cerevisiae and malolactic bacteria: preliminary characterization of a yeast proteinaceous compound(s) active against Oenococcus oeni , 2005, Journal of applied microbiology.

[8]  M. Constantí,et al.  Population dynamics of Oenococcus oeni strains in a new winery and the effect of SO2 and yeast strain. , 2005, FEMS microbiology letters.

[9]  H. Alexandre,et al.  Saccharomyces cerevisiae-Oenococcus oeni interactions in wine: current knowledge and perspectives. , 2004, International journal of food microbiology.

[10]  G. Fia,et al.  Influence of different pH values and inoculation time on the growth and malolactic activity of a strain of Oenococcus oeni , 2003 .

[11]  P. Henschke,et al.  Interaction of pH, ethanol concentration and wine matrix on induction of malolactic fermentation with commercial ‘direct inoculation’ starter cultures , 2003 .

[12]  M. Viljoen-Bloom,et al.  Differential malic acid degradation by selected strains of Saccharomyces during alcoholic fermentation. , 2003, International journal of food microbiology.

[13]  D. Torrea,et al.  Content of biogenic amines in a Chardonnay wine obtained through spontaneous and inoculated fermentations. , 2002, Journal of agricultural and food chemistry.

[14]  M. Constantí,et al.  Inhibitory effect of sulfur dioxide and other stress compounds in wine on the ATPase activity of Oenococcus oeni. , 2002, FEMS microbiology letters.

[15]  A. Lonvaud-Funel Biogenic amines in wines: role of lactic acid bacteria. , 2001, FEMS microbiology letters.

[16]  C. G. Edwards,et al.  Inducing malolactic fermentation in Chardonnay musts and wines using different strains of Oenococcus oeni , 2001 .

[17]  C. Antolín,et al.  Influence of Training System on the Reproductive Development and Hormonal Levels of Vitis vinifera L. cv. Tempranillo , 2001 .

[18]  J. Osborne,et al.  Acetaldehyde metabolism by wine lactic acid bacteria. , 2000, FEMS microbiology letters.

[19]  C. Libertun,et al.  AN IMPROVED HPLC METHOD FOR IDENTIFICATION AND QUANTITATION OF POLYAMINES AND RELATED COMPOUNDS AS BENZOYLATED DERIVATIVES , 2000 .

[20]  G. Revel,et al.  Evaluation of the settling of malolactic starters within the indigenous microflora of wines , 1997 .

[21]  K. Fugelsang Fermentation and Post-fermentation Processing , 1997 .

[22]  L. Gény,et al.  Effect of excessive nitrogen nutrition on free and conjugated polyamines content of leaves and berries of Vitis vinifera L. during their development , 1996 .

[23]  J. C. Peterson,et al.  Relationship Between Sluggish Fermentations and the Antagonism of Yeast by Lactic Acid Bacteria , 1996, American Journal of Enology and Viticulture.

[24]  A. Lonvaud-Funel,et al.  Malolactic Fermentation in Wine by Direct Inoculation with Freeze-Dried Leuconostoc oenos Cultures , 1996, American Journal of Enology and Viticulture.

[25]  P. Iland,et al.  Techniques for chemical analysis and stability tests of grape juice and wine , 1993 .

[26]  A. Joyeux,et al.  Inhibition of malolactic fermentation of wines by products of yeast metabolism , 1988 .

[27]  S. W. King,et al.  Metabolic Interactions BetweenSaccharomyces cerevisiaeandLeuconostoc oenosin a Model Grape Juice/Wine System , 1986, American Journal of Enology and Viticulture.

[28]  T. H. Lee,et al.  Practical Implications of Malolactic Fermentation: A Review , 1985, American Journal of Enology and Viticulture.