Highly Stable Core-Shell Nanocolloids: Synergy between Nano-Silver and Natural Polymers to Prevent Biofilm Formation

Active investment in research time in the development and study of novel unconventional antimicrobials is trending for several reasons. First, it is one of the ways which might help to fight antimicrobial resistance and bacterial contamination due to uncontrolled biofilm growth. Second, minimizing harmful environmental outcomes due to the overuse of toxic chemicals is one of the highest priorities nowadays. We propose the application of two common natural compounds, chitosan and tannic acid, for the creation of a highly crosslinked polymer blend with not only intrinsic antimicrobial properties but also reducing and stabilizing powers. Thus, the fast and green synthesis of fine spherically shaped Ag nanoparticles and further study of the composition and properties of the colloids took place. A positively charged core-shell nanocomposition, with an average size in terms of the metal core of 17 ± 4 nm, was developed. Nanoantimicrobials were characterized by several spectroscopic (UV-vis and FTIR) and microscopic (transmission and scanning electron microscopies) techniques. The use of AgNPs as a core and an organic polymer blend as a shell potentially enable a synergistic long-lasting antipathogen effect. The antibiofilm potential was studied against the food-borne pathogens Salmonella enterica and Listeria monocytogenes. The antibiofilm protocol efficiency was evaluated by performing crystal violet assay and optical density measurements, direct visualization by confocal laser scanning microscopy and morphological studies by SEM. It was found that the complex nanocomposite has the ability to prevent the growth of biofilm. Further investigation for the potential application of this stable composition in food packaging will be carried out.

[1]  Xuerui Bao,et al.  Strategies for controlling biofilm formation in food industry , 2022, Grain & Oil Science and Technology.

[2]  Samy M. Shaban,et al.  Fabrication of activated carbon fiber functionalized core–shell silver nanoparticles based in situ and low-cost technology for wound dressings with an enhanced antimicrobial activity and cell viability , 2022, Journal of Molecular Liquids.

[3]  V. Gundabala,et al.  Antimicrobial bio-inspired active packaging materials for shelf life and safety development: A review , 2022, Food Bioscience.

[4]  W. Elkhatib,et al.  Antibiofilm activity of green synthesized silver nanoparticles against biofilm associated enterococcal urinary pathogens , 2022, Scientific Reports.

[5]  Junling Shi,et al.  Advantages of silver nanoparticles synthesized by microorganisms in antibacterial activity , 2022, Green Synthesis of Silver Nanomaterials.

[6]  Masoud Delfi,et al.  Advances in tannic acid-incorporated biomaterials: Infection treatment, regenerative medicine, cancer therapy, and biosensing , 2021, Chemical Engineering Journal.

[7]  L. Landeros-Martínez,et al.  Antimicrobial Resistance and Inorganic Nanoparticles , 2021, International journal of molecular sciences.

[8]  S. Jafari,et al.  Chitosan-based nanodelivery systems for cancer therapy: Recent advances. , 2021, Carbohydrate polymers.

[9]  G. Sotiriou,et al.  Antibiofilm activity of nanosilver coatings against Staphylococcus aureus. , 2021, Journal of colloid and interface science.

[10]  A. Maleki,et al.  Fe3O4@chitosan-tannic acid bionanocomposite as a novel nanocatalyst for the synthesis of pyranopyrazoles , 2021, Scientific Reports.

[11]  I. Aranaz,et al.  Chitosan: An Overview of Its Properties and Applications , 2021, Polymers.

[12]  F. Chen,et al.  Tannic Acid: A green and efficient stabilizer of Au, Ag, Cu and Pd nanoparticles for the 4-Nitrophenol Reduction, Suzuki-Miyaura coupling reactions and click reactions in aqueous solution. , 2021, Journal of colloid and interface science.

[13]  Syed Imdadul Hossain,et al.  Ag-Based Synergistic Antimicrobial Composites. A Critical Review , 2021, Nanomaterials.

[14]  S. Matroodi,et al.  Synergistic effects of combinatorial chitosan and polyphenol biomolecules on enhanced antibacterial activity of biofunctionalaized silver nanoparticles , 2020, Scientific Reports.

[15]  N. Hüsing,et al.  Tannin-Based Hybrid Materials and Their Applications: A Review , 2020, Molecules.

[16]  R. Bonomo,et al.  Overview: The Ongoing Threat of Antimicrobial Resistance. , 2020, Infectious disease clinics of North America.

[17]  R. Karaman,et al.  Resistance of Gram-Positive Bacteria to Current Antibacterial Agents and Overcoming Approaches , 2020, Molecules.

[18]  J. Rhim,et al.  Chitosan-based biodegradable functional films for food packaging applications , 2020 .

[19]  Scott G. Mitchell,et al.  Biofilm Eradication Using Biogenic Silver Nanoparticles , 2020, Molecules.

[20]  Z. A. Raza,et al.  Recent developments in chitosan encapsulation of various active ingredients for multifunctional applications. , 2020, Carbohydrate research.

[21]  Jianxin Zhao,et al.  The physicochemical properties of chitosan prepared by microwave heating , 2020, Food science & nutrition.

[22]  I. C. Tessaro,et al.  Impact of acid type and glutaraldehyde crosslinking in the physicochemical and mechanical properties and biodegradability of chitosan films , 2020, Polymer Bulletin.

[23]  E. Drăgan,et al.  Advances in porous chitosan-based composite hydrogels: Synthesis and applications , 2020 .

[24]  F. Cappitelli,et al.  Testing Anti-Biofilm Polymeric Surfaces: Where to Start? , 2019, International journal of molecular sciences.

[25]  G. Martínez-Castañón,et al.  Molecular Mechanisms of Bacterial Resistance to Metal and Metal Oxide Nanoparticles , 2019, International journal of molecular sciences.

[26]  Asad U. Khan,et al.  Antibiotics versus biofilm: an emerging battleground in microbial communities , 2019, Antimicrobial Resistance & Infection Control.

[27]  Hao Wu,et al.  Double Cross-Linked Chitosan Composite Films Developed with Oxidized Tannic Acid and Ferric Ions Exhibit High Strength and Excellent Water Resistance. , 2019, Biomacromolecules.

[28]  M. Jesús,et al.  An overview of the chemical modifications of chitosan and their advantages , 2018 .

[29]  N. Reddy,et al.  Crosslinked chitosan films with controllable properties for commercial applications. , 2018, International journal of biological macromolecules.

[30]  M. Krzyżowska,et al.  Antiviral Activity of Tannic Acid Modified Silver Nanoparticles: Potential to Activate Immune Response in Herpes Genitalis , 2018, Viruses.

[31]  Sabu Thomas,et al.  Biopolymer based nanomaterials in drug delivery systems: A review , 2018, Materials Today Chemistry.

[32]  A. Kędziora,et al.  Similarities and Differences between Silver Ions and Silver in Nanoforms as Antibacterial Agents , 2018, International journal of molecular sciences.

[33]  M. Moloto,et al.  Green synthesis of chitosan capped silver nanoparticles and their antimicrobial activity , 2018 .

[34]  P. Guerrero,et al.  Chitosan as a bioactive polymer: Processing, properties and applications. , 2017, International journal of biological macromolecules.

[35]  R. D. Vasquez,et al.  Polysaccharide-mediated green synthesis of silver nanoparticles from Sargassum siliquosum J.G. Agardh: Assessment of toxicity and hepatoprotective activity , 2016 .

[36]  G. I. Godahewa,et al.  Antimicrobial effects of chitosan silver nano composites (CAgNCs) on fish pathogenic Aliivibrio (Vibrio) salmonicida , 2016 .

[37]  Jian-Jun Li,et al.  Tuning the shell thickness-dependent plasmonic absorption of Ag coated Au nanocubes: The effect of synthesis temperature , 2015 .

[38]  Narendra Reddy,et al.  Crosslinking biopolymers for biomedical applications. , 2015, Trends in biotechnology.

[39]  Praveena Nair,et al.  Physical and chemical reinforcement of chitosan film using nanocrystalline cellulose and tannic acid , 2015, Cellulose.

[40]  Amr T. M. Saeb,et al.  A Review on Antimicrobial Chitosan-Silver Nanocomposites: A Roadmap Toward Pathogen Targeted Synthesis , 2015 .

[41]  Dinesh Kumar,et al.  Nanoparticles and core–shell nanocomposite based new generation water remediation materials and analytical techniques: A review , 2014 .

[42]  D. Fernig,et al.  A rapid method to estimate the concentration of citrate capped silver nanoparticles from UV-visible light spectra. , 2014, The Analyst.

[43]  Piotr Orlowski,et al.  Tannic Acid Modified Silver Nanoparticles Show Antiviral Activity in Herpes Simplex Virus Type 2 Infection , 2014, PloS one.

[44]  A. Subramanian,et al.  Consequences of Neutralization on the Proliferation and Cytoskeletal Organization of Chondrocytes on Chitosan-Based Matrices , 2011 .

[45]  A. Pinotti,et al.  Crosslinking capacity of tannic acid in plasticized chitosan films , 2010 .

[46]  V. Mourya,et al.  Trimethyl chitosan and its applications in drug delivery , 2009, Journal of materials science. Materials in medicine.

[47]  G. O’Toole,et al.  Mechanisms of biofilm resistance to antimicrobial agents. , 2001, Trends in microbiology.