Light-induced release of DNA from gold nanoparticles: nanoshells and nanorods.

Plasmon-resonant nanoparticle complexes show highly promising potential for light-triggered, remote-controlled delivery of oligonucleotides on demand, for research and therapeutic purposes. Here we investigate the light-triggered release of DNA from two types of nanoparticle substrates: Au nanoshells and Au nanorods. Both light-triggered and thermally induced release are distinctly observable from nanoshell-based complexes, with light-triggered release occurring at an ambient solution temperature well below the DNA melting temperature. Surprisingly, no analogous measurable release was observable from nanorod-based complexes below the DNA melting temperature. These results suggest that a nonthermal mechanism may play a role in plasmon resonant, light-triggered DNA release.

[1]  Hironobu Takahashi,et al.  Controlled release of plasmid DNA from gold nanorods induced by pulsed near-infrared light. , 2005, Chemical communications.

[2]  Glenn P. Goodrich,et al.  Plasmonic enhancement of molecular fluorescence. , 2007, Nano letters.

[3]  Scott C. Brown,et al.  Nanoparticles for bioimaging. , 2006, Advances in colloid and interface science.

[4]  Wei Zhang,et al.  Gold nanoparticle ensembles as heaters and actuators: melting and collective plasmon resonances , 2006, Nanoscale Research Letters.

[5]  Naomi J. Halas,et al.  Photodetection with Active Optical Antennas , 2011, Science.

[6]  Dipankar Sen,et al.  Photothermal release of single-stranded DNA from the surface of gold nanoparticles through controlled denaturating and Au-S bond breaking. , 2010, ACS nano.

[7]  Sabine Neuss,et al.  Gold nanoparticles of diameter 1.4 nm trigger necrosis by oxidative stress and mitochondrial damage. , 2009, Small.

[8]  Robia G. Pautler,et al.  Tracking of multimodal therapeutic nanocomplexes targeting breast cancer in vivo. , 2010, Nano letters.

[9]  Naomi J Halas,et al.  Nanoshells made easy: improving Au layer growth on nanoparticle surfaces. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[10]  L. Novotný,et al.  Enhancement and quenching of single-molecule fluorescence. , 2006, Physical review letters.

[11]  M. Steigerwald,et al.  Plasmon Induced Photovoltage and Charge Separation in Citrate-Stabilized Gold Nanoparticles , 2010 .

[12]  Melting transition of directly linked gold nanoparticle DNA assembly , 2005, physics/0503090.

[13]  K. Hamad-Schifferli,et al.  Ligand customization and DNA functionalization of gold nanorods via round-trip phase transfer ligand exchange. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[14]  Matthew Tirrell,et al.  Laser-Activated Gene Silencing via Gold Nanoshell-siRNA Conjugates. , 2009, ACS nano.

[15]  Xiaohua Huang,et al.  Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: applications in oral cancer. , 2005, Nano letters.

[16]  A. Nitzan,et al.  Can photochemistry be enhanced on rough surfaces , 1981 .

[17]  Luke P. Lee,et al.  Remote optical switch for localized and selective control of gene interference. , 2009, Nano letters.

[18]  Younan Xia,et al.  Gold nanocages: synthesis, properties, and applications. , 2008, Accounts of chemical research.

[19]  T. Niidome,et al.  Controlled-release system of single-stranded DNA triggered by the photothermal effect of gold nanorods and its in vivo application. , 2011, Bioorganic & medicinal chemistry.

[20]  Leon Hirsch,et al.  Gold nanoshell bioconjugates for molecular imaging in living cells. , 2005, Optics letters.

[21]  Romain Quidant,et al.  Nanoscale control of optical heating in complex plasmonic systems. , 2010, ACS nano.

[22]  Scott C. Brown,et al.  Nanoparticles as contrast agents for in-vivo bioimaging: current status and future perspectives , 2011, Analytical and bioanalytical chemistry.

[23]  L. Brus.  Noble metal nanocrystals: plasmon electron transfer photochemistry and single-molecule Raman spectroscopy. , 2008, Accounts of chemical research.

[24]  Alexander O. Govorov,et al.  Generating heat with metal nanoparticles , 2007 .

[25]  Hui Zhang,et al.  Gold nanocages: bioconjugation and their potential use as optical imaging contrast agents. , 2005, Nano letters.

[26]  Naomi J. Halas,et al.  Nanoengineering of optical resonances , 1998 .

[27]  A. Govorov,et al.  Experimental and theoretical studies of light-to-heat conversion and collective heating effects in metal nanoparticle solutions. , 2009, Nano letters.

[28]  C. Mirkin,et al.  Photoinduced Conversion of Silver Nanospheres to Nanoprisms , 2001, Science.

[29]  L. Brus,et al.  “Hot Electron” Photo-Charging and Electrochemical Discharge Kinetics of Silver Nanocrystals , 2007 .

[30]  Emil Prodan,et al.  Quantum plasmonics: optical properties and tunability of metallic nanorods. , 2010, ACS nano.

[31]  B. MacCraith,et al.  Optimization of Nanoparticle Size for Plasmonic Enhancement of Fluorescence , 2007 .

[32]  Yi-Cheng Chen,et al.  DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation. , 2006, Journal of the American Chemical Society.

[33]  Stephan Link,et al.  Optical properties and ultrafast dynamics of metallic nanocrystals. , 2003, Annual review of physical chemistry.

[34]  Romain Quidant,et al.  Heat generation in plasmonic nanostructures: Influence of morphology , 2009 .

[35]  K. Hamad-Schifferli,et al.  Selective release of multiple DNA oligonucleotides from gold nanorods. , 2009, ACS nano.

[36]  Younan Xia,et al.  Metal Nanostructures with Hollow Interiors , 2003 .

[37]  Tammy Y. Olson,et al.  Synthesis, characterization, and tunable optical properties of hollow gold nanospheres. , 2006, The journal of physical chemistry. B.

[38]  Glenn P. Goodrich,et al.  Photothermal Efficiencies of Nanoshells and Nanorods for Clinical Therapeutic Applications , 2009 .

[39]  Wei Lu,et al.  In vitro and in vivo targeting of hollow gold nanoshells directed at epidermal growth factor receptor for photothermal ablation therapy , 2008, Molecular Cancer Therapeutics.

[40]  Li Cd,et al.  Photoinduced Electron Transfer at Molecule−Metal Interfaces , 2006 .

[41]  Louis E. Brus,et al.  Silver Nanodisk Growth by Surface Plasmon Enhanced Photoreduction of Adsorbed [Ag+] , 2003 .

[42]  C. Mirkin,et al.  A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles. , 2000, Analytical chemistry.

[43]  Sabine Neuss,et al.  Size-dependent cytotoxicity of gold nanoparticles. , 2007, Small.

[44]  R. Weissleder.  A clearer vision for in vivo imaging , 2001, Nature Biotechnology.

[45]  R. W. Christy,et al.  Optical Constants of the Noble Metals , 1972 .

[46]  P. Nordlander,et al.  A Hybridization Model for the Plasmon Response of Complex Nanostructures , 2003, Science.

[47]  A. Nitzan,et al.  Theoretical model for enhanced photochemistry on rough surfaces , 1981 .

[48]  Benjamin Thierry,et al.  Drug nanocarriers and functional nanoparticles: applications in cancer therapy. , 2009, Current drug delivery.

[49]  Wah Chiu,et al.  Remotely triggered liposome release by near-infrared light absorption via hollow gold nanoshells. , 2008, Journal of the American Chemical Society.

[50]  Naomi J. Halas,et al.  Light-induced release of DNA from plasmon-resonant nanoparticles: Towards light-controlled gene therapy , 2009 .

[51]  Li Jiang,et al.  Gold hollow nanospheres: tunable surface plasmon resonance controlled by interior-cavity sizes. , 2005, The journal of physical chemistry. B.

[52]  Frank Bridges,et al.  Near infrared optical absorption of gold nanoparticle aggregates , 2002 .

[53]  Catherine J Murphy,et al.  Seeded high yield synthesis of short Au nanorods in aqueous solution. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[54]  Vahid Sandoghdar,et al.  Enhancement of single-molecule fluorescence using a gold nanoparticle as an optical nanoantenna. , 2006, Physical review letters.

[55]  Vincent M Rotello,et al.  Gold nanoparticles in delivery applications. , 2008, Advanced drug delivery reviews.

[56]  Catherine J. Murphy,et al.  Wet Chemical Synthesis of High Aspect Ratio Cylindrical Gold Nanorods , 2001 .

[57]  Kaylie L. Young,et al.  Plasmonically controlled nucleic acid dehybridization with gold nanoprisms. , 2009, Chemphyschem : a European journal of chemical physics and physical chemistry.