Structural Studies of Induced Antibodies with Defined Idiotypic Specificities

The heavy (H) chains of three anti‐streptococcal group A carbohydrate antibodies (anti‐SACHO) of restricted heterogeneity, elicited in inbred Sprague‐Dawley rats, were subjected to 40 cycles of automated Edman degradation. All three antibody H‐chain preparations had an identical amino acid sequence and were classified as a variant of the VHIII subgroup of immunoglobulin H chains. A comparison of the H‐chain sequence of these antibodies with that of pooled rat gamma chains suggests that a very minor population of H chains (closely related to the VHIII subgroup) has been ‘recruited’ as a consequence of this immunization. This observation further supports the notion that ‘framework’ and ‘specificity’ or “hypervariable regions” are closely linked.

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