Expression of Insulin-like Growth Factor (IGF)-Il and IGF-I Receptor during Proliferation and Differentiation of

We have studied the expression of insulin-like growth fador type II (IGF-ll) and its autocrine role during the proliferation and differentiation of the CaCo-2 colon carcinoma cell line. ICE-Il RNA levels were high in proliferating cells and decreased by more than 10-fold when cells ceased to proliferate and differentiated. Immunoreadive ICE-Il protein was high in the conditioned media of proliferating cells and decreased 20-fold in the media of differentiated cells. Reduced ICE-Il expression was associated with a decrease in ICE-I receptor number that was high in proliferating cells (approximately 80,000 binding sites/cell) and reduced by 4-fold in differentiated cells. Exogenously added ICE-Il was able to stimulate proliferation of serum-deprived cells in a dose-dependent fashion. ICE-Il aded through the ICE-I receptor, since both basal and ICE-Il-stimulated cell proliferation was inhibited by the monoclonal antibody a-lR3, which blocks the binding sites of the ICE-I receptor. The inhibition of CaCo-2 basal cell growth by the a-lR3 antibody suggeststhat ICE-lI may ad as an autocrine growth fador for these cells.

[1]  Y. Lee,et al.  Inhibition of protein phosphatases activates P4 promoter of the human insulin-like growth factor II gene through the specific promoter element. , 1994, Journal of Biological Chemistry.

[2]  J. Baker,et al.  Mice carrying null mutations of the genes encoding insulin-like growth factor I (Igf-1) and type 1 IGF receptor (Igf1r) , 1993, Cell.

[3]  K. Lee,et al.  Dibutyryl cyclic adenosine monophosphate differentially regulates cell proliferation in low and high alkaline phosphatase SaOS‐2 human osteosarcoma cells: Evidence for mediation by the insulin‐like growth factor‐II system , 1993, Journal of cellular physiology.

[4]  J. Gordon.  Understanding gastrointestinal epithelial cell biology: lessons from mice with help from worms and flies. , 1993, Gastroenterology.

[5]  T. Hughes,et al.  Increased expression of apolipoprotein genes accompanies differentiation in the intestinal cell line Caco-2. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[6]  R. Benya,et al.  Extracellular matrix composition influences insulinlike growth factor I receptor expression in rat IEC-18 cells. , 1993, Gastroenterology.

[7]  R. McCusker,et al.  Secretion of insulin-like growth factor II (IGF-II) and IGF-binding protein-2 by intestinal epithelial (IEC-6) cells: implications for autocrine growth regulation. , 1992, Endocrinology.

[8]  J. Marvaldi,et al.  Expression of type I, but not type II insulin-like growth factor receptor on both undifferentiated and differentiated HT29 human colon carcinoma cell line. , 1992, The Journal of clinical endocrinology and metabolism.

[9]  V. Macaulay,et al.  Insulin-like growth factors and cancer. , 1992, British Journal of Cancer.

[10]  W. Karnes,et al.  Autonomous proliferation of colon cancer cells that coexpress transforming growth factor α and its receptor , 1992 .

[11]  Jun Kato,et al.  Binding specificities and transducing function of the different molecular weight forms of insulin-like growth factor-II (IGF-II) on IGF-I receptors. , 1991, Endocrinology.

[12]  J R Florini,et al.  "Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. , 1991, The Journal of biological chemistry.

[13]  D. Podolsky,et al.  Regulation of transforming growth factor expression in rat intestinal epithelial cell lines. , 1991, The Journal of clinical investigation.

[14]  A. Brunetti,et al.  Regulation of insulin-like growth factor (IGF) I receptor expression during muscle cell differentiation. Potential autocrine role of IGF-II. , 1991, The Journal of clinical investigation.

[15]  S. Steinberg,et al.  Insulin-like growth factor II-mediated proliferation of human neuroblastoma. , 1991, The Journal of clinical investigation.

[16]  S. Gammeltoft,et al.  Translational discrimination of mRNAs coding for human insulin-like growth factor II. , 1990, The Journal of biological chemistry.

[17]  R. Humbel,et al.  Insulin-like growth factors I and II. , 1990, European journal of biochemistry.

[18]  J. Vanderhoof,et al.  Characterization of type I and type II insulin-like growth factor receptors in an intestinal epithelial cell line. , 1990, Endocrinology.

[19]  J. Fantini,et al.  Production of insulin‐like growth factor II (IGF‐II) and different forms of IGF‐binding proteins by HT‐29 human colon carcinoma cell line , 1990, Journal of cellular physiology.

[20]  R. Whitehead,et al.  Autocrine regulation of human tumor cell proliferation by insulin-like growth factor II: an in-vitro model. , 1990, Endocrinology.

[21]  A. Efstratiadis,et al.  A growth-deficiency phenotype in heterozygous mice carrying an insulin-like growth factor II gene disrupted by targeting , 1990, Nature.

[22]  S. Gammeltoft,et al.  Expression of two types of receptor for insulinlike growth factors in human colonic epithelium. , 1990, Gastroenterology.

[23]  C. Osborne,et al.  Insulin-like growth factor-II (IGF-II): a potential autocrine/paracrine growth factor for human breast cancer acting via the IGF-I receptor. , 1989, Molecular endocrinology.

[24]  P. Tagliaferri,et al.  Ornithine decarboxylase and diamine oxidase in human colon carcinoma cell line CaCo-2 in culture. , 1989, Gastroenterology.

[25]  P. Rotwein,et al.  Coordinate expression of insulin-like growth factor II and its receptor during muscle differentiation. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[26]  O. Asami,et al.  Identification of a family of insulin-like growth factor II secreted by cultured rat epithelial-like cell line 18,54-SF: application of a monoclonal antibody. , 1989, Endocrinology.

[27]  J. Minna,et al.  Insulin-like growth factor-I can mediate autocrine proliferation of human small cell lung cancer cell lines in vitro. , 1988, The Journal of clinical investigation.

[28]  S. Gammeltoft,et al.  Receptors for insulin-like growth factors I and II in rat gastrointestinal epithelium. , 1988, The American journal of physiology.

[29]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[30]  T. Shows,et al.  Enhanced levels of insulin-like growth factor messenger RNA in human colon carcinomas and liposarcomas. , 1986, Cancer research.

[31]  P. Usher,et al.  Monoclonal antibody to the type I insulin-like growth factor (IGF-I) receptor blocks IGF-I receptor-mediated DNA synthesis: clarification of the mitogenic mechanisms of IGF-I and insulin in human skin fibroblasts. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[32]  J. Erickson,et al.  Variation among human 28S ribosomal RNA genes. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[33]  G A McPherson,et al.  Analysis of radioligand binding experiments. A collection of computer programs for the IBM PC. , 1985, Journal of pharmacological methods.

[34]  J. Breslow,et al.  Isolation and characterization of the human apolipoprotein A-I gene. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[35]  D Rodbard,et al.  Ligand: a versatile computerized approach for characterization of ligand-binding systems. , 1980, Analytical biochemistry.

[36]  M. Eccles,et al.  Structural analysis of the human insulin-like growth factor-II P3 promoter. , 1993, The Biochemical journal.

[37]  E. Harlow,et al.  Antibodies: A Laboratory Manual , 1988 .

[38]  S. Povey,et al.  Isolation of a cDNA probe for a human jejunal brush-border hydrolase, sucrase-isomaltase, and assignment of the gene locus to chromosome 3. , 1987, Gene.

[39]  G. Bell,et al.  Sequence of a cDNA clone encoding human preproinsulin-like growth factor II , 1984, Nature.

[40]  M. Pinto,et al.  Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture , 1983 .