An Outbreak of Aeromonas Hydrophila Food Poisoning in Deptsang Village, Samdrup Jongkhar, Bhutan, 2016

Background: An outbreak investigation was carried out to determine the cause and confirm the source of food poisoning in Deptsang village for implementing prevention and control measures. Methods: We conducted a retrospective cohort study for the outbreak investigation. Stool specimens were collected from cases to perform culture and antibiogram. The team also inspected the environment and hygiene practices in both the construction site and the entire community. The association between the exposure to carcass meat and their outcome of acute gastroenteritis was assessed by risk ratio. P<0.05 was considered statistically significant. Results: Fifty-five villagers consumed the carcass meat during lunch and dinner resulting in 33 cases. Multi-drug resistant Aeromonas hydrophila was isolated from stool specimens of cases, which were susceptible to chloramphenicol only. A risk ratio of 2.1 was found between those people who consumed the carcass meat and those who did not consume the carcass meat (P<0.001). Conclusions: The current outbreak of food poisoning was caused by the consumption of carcass meat contaminated with A. hydrophila. Provision of health education with emphasis on food hygiene is needed in remote areas to prevent such outbreaks in the future.

[1]  M. S. Soltan Dallal,et al.  Prevalence, virulence and antimicrobial resistance patterns of Aeromonas spp. isolated from children with diarrhea. , 2016, Germs.

[2]  S. Shekhar,et al.  Incidence of Aeromonas spp. infection in fish and chicken meat and its related public health hazards: A review , 2016, Veterinary world.

[3]  E. Silbergeld,et al.  Risky Food Safety Behaviors Are Associated with Higher Bmi and Lower Healthy Eating Self-Efficacy and Intentions among African American Churchgoers in Baltimore , 2012, PloS one.

[4]  A. Okoh,et al.  Antibiotic Susceptibility Profile of Aeromonas Species Isolated from Wastewater Treatment Plant , 2012, TheScientificWorldJournal.

[5]  E. Igumbor,et al.  Emerging Aeromonas Species Infections and Their Significance in Public Health , 2012, TheScientificWorldJournal.

[6]  R. Berg,et al.  Distribution of Virulence Factors and Molecular Fingerprinting of Aeromonas Species Isolates from Water and Clinical Samples: Suggestive Evidence of Water-to-Human Transmission , 2010, Applied and Environmental Microbiology.

[7]  S. Abbott,et al.  The Genus Aeromonas: Taxonomy, Pathogenicity, and Infection , 2010, Clinical Microbiology Reviews.

[8]  S. Echeverrigaray,et al.  Aeromonas associated diarrhoeal disease in south Brazil: prevalence, virulence factors and antimicrobial resistance , 2007 .

[9]  A. Samie,et al.  Prevalence, Pathogenesis, Antibiotic Susceptibility Profiles, and In-vitro Activity of Selected Medicinal Plants Against Aeromonas Isolates from Stool Samples of Patients in the Venda Region of South Africa , 2007, Journal of health, population, and nutrition.

[10]  Gillian Hall,et al.  Estimating the burden of acute gastroenteritis, foodborne disease, and pathogens commonly transmitted by food: an international review. , 2005, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[11]  M. Ferraro.  Performance standards for antimicrobial susceptibility testing , 2001 .

[12]  P. Caumette,et al.  Impact of an Urban Effluent on Antibiotic Resistance of Riverine Enterobacteriaceae andAeromonas spp , 2000, Applied and Environmental Microbiology.

[13]  R. Walker.  Standards for antimicrobial susceptibility testing. , 1999, American journal of veterinary research.

[14]  M. Handfield,et al.  Aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29) , 1996, Applied and environmental microbiology.