PAH exposure and oxidative stress indicators of human cohorts exposed to traffic pollution in Lahore city (Pakistan).

Pollution from road traffic is not only a major source of exposure to polycyclic aromatic hydrocarbons (PAHs) but also a growing problem in the city of Lahore (Pakistan). In this study, we evaluated exposure to traffic-related PAHs, among subjects including traffic police officers (TPs), rickshaw drivers (RKs) shopkeepers working near main roads (SKs) and a control group (CN) for comparative analyses. We monitored the 1-hydroxypyrene (1-OHP) as biomarkers of exposure to PAHs and its probable association with catalase (CAT), glutathione (GSH) glutathione peroxidase (GSHPx) activity as biomarkers of oxidative stress in selected cohorts from the city Lahore. Results showed that median 1-OHP concentration was significantly higher in TPs than CN (med 1.21 vs. 0.51 μmol mol-C(-1) respectively, P=0.046), followed by RKs (0.68 μmol mol-C(-1), P=0.19 vs. CN). Furthermore, GSH, GSHPx, and CAT activities were also higher in exposed subjects than CN, which indicated that they experienced oxidative stress. Similar, but less severe observations were recorded in SKs. Observation of self-reported health status showed that, on the basis of daily time spent in the middle of heavy traffic, TPs and RKs most frequently suffered from adverse head and respiratory symptoms. The study shows that increasing traffic pollution can be associated with important health risk factor not only for the workers in transport industry but also for the public. Finally, the issue of traffic pollution in Lahore city needs to be addressed on priority.

[1]  L. Flohé,et al.  Assays of glutathione peroxidase. , 1984, Methods in enzymology.

[2]  Antonio F. Hernández,et al.  Evaluation of pesticide-induced oxidative stress from a gene-environment interaction perspective. , 2013, Toxicology.

[3]  Szu-Chieh Chen,et al.  Lung cancer risk in relation to traffic-related nano/ultrafine particle-bound PAHs exposure: a preliminary probabilistic assessment. , 2011, Journal of hazardous materials.

[4]  E. Elovaara,et al.  Simultaneous analysis of naphthols, phenanthrols, and 1-hydroxypyrene in urine as biomarkers of polycyclic aromatic hydrocarbon exposure: intraindividual variance in the urinary metabolite excretion profiles caused by intervention with β-naphthoflavone induction in the rat , 2003, Archives of Toxicology.

[5]  Ole Raaschou-Nielsen,et al.  Urinary 1-hydroxypyrene in children living in city and rural residences in Denmark. , 2005, The Science of the total environment.

[6]  Xiaochuan Pan,et al.  Exposures to Particulate Matter and Polycyclic Aromatic Hydrocarbons and Oxidative Stress in Schoolchildren , 2009, Environmental health perspectives.

[7]  X. Wang,et al.  Genotoxicity and chronic health effects of automobile exhaust: a study on the traffic policemen in the city of Lanzhou. , 1998, Mutation research.

[8]  Atif Kamal,et al.  Chemical exposure in occupational settings and related health risks: a neglected area of research in Pakistan. , 2012, Environmental toxicology and pharmacology.

[9]  M. Athar,et al.  Impact of transport and industrial emissions on the ambient air quality of Lahore City, Pakistan , 2010, Environmental monitoring and assessment.

[10]  M. Kandefer-Szerszeń,et al.  Accelerated apoptosis of blood leukocytes and oxidative stress in blood of patients with major depression , 2008, Progress in Neuro-Psychopharmacology and Biological Psychiatry.

[11]  F. Sánchez-Jiménez,et al.  Antioxidant enzymes and their implications in pathophysiologic processes. , 1999, Frontiers in bioscience : a journal and virtual library.

[12]  Omar Masera,et al.  Improved biomass stove intervention in rural Mexico: impact on the respiratory health of women. , 2009, American journal of respiratory and critical care medicine.

[13]  M. Shao,et al.  Personal exposure to particulate PAHs and anthraquinone and oxidative DNA damages in humans. , 2010, Chemosphere.

[14]  T. Sura,et al.  The influence of metabolic gene polymorphisms on urinary 1-hydroxypyrene concentration in Thai bus drivers. , 2011, Environmental toxicology and pharmacology.

[15]  I. Ferreira,et al.  A review on antioxidants, prooxidants and related controversy: natural and synthetic compounds, screening and analysis methodologies and future perspectives. , 2013, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[16]  Kazuhiko Ito,et al.  Fine Particulate Matter Constituents Associated with Cardiovascular Hospitalizations and Mortality in New York City , 2010, Environmental health perspectives.

[17]  P. Gosset,et al.  Antioxidant defense disruption by polycyclic aromatic hydrocarbons-coated onto Fe(2)O(3) particles in human lung cells (A549). , 2001, Toxicology.

[18]  Z. Bai,et al.  Health risk assessment for traffic policemen exposed to polycyclic aromatic hydrocarbons (PAHs) in Tianjin, China. , 2007, The Science of the total environment.

[19]  F Merlo,et al.  Urinary excretion of 1-hydroxypyrene as a marker for exposure to urban air levels of polycyclic aromatic hydrocarbons. , 1998, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[20]  Huilian Zhu,et al.  Elevated Oxidative Damage in Kitchen Workers in Chinese Restaurants , 2011, Journal of occupational health.

[21]  T. Sandström,et al.  Antioxidant airway responses following experimental exposure to wood smoke in man , 2010, Particle and Fibre Toxicology.

[22]  Nghiem Trung Dung,et al.  Emission of Polycyclic Aromatic Hydrocarbons and Particulate Matter from Domestic Combustion of Selected Fuels , 1999 .

[23]  Jyoti,et al.  Blood levels of polycyclic aromatic hydrocarbons in children and their association with oxidative stress indices: an Indian perspective. , 2008, Clinical biochemistry.

[24]  Atif Kamal,et al.  Biological Monitoring of Blood Naphthalene Levels as a Marker of Occupational Exposure to PAHs among Auto-Mechanics and Spray Painters in Rawalpindi , 2011, BMC public health.

[25]  C. Chuang,et al.  Urinary 1‐Hydroxypyrene Level Relative to Vehicle Exhaust Exposure Mediated by Metabolic Enzyme Polymorphisms , 2007, Journal of occupational health.

[26]  P. Apostoli,et al.  Environmental and biological monitoring of benzene exposure in a cohort of Italian taxi drivers. , 2006, Toxicology letters.

[27]  J. Morrow,et al.  Biomarker responses and disease susceptibility in juvenile rainbow trout Oncorhynchus mykiss fed a high molecular weight PAH mixture , 2011, Environmental toxicology and chemistry.

[28]  A. Zuckerman,et al.  IARC Monographs on the Evaluation of Carcinogenic Risks to Humans , 1995, IARC monographs on the evaluation of carcinogenic risks to humans.

[29]  Suman Sharma,et al.  Measurement of genotoxic air pollutant exposures in street vendors and school children in and near Bangkok. , 2005, Toxicology and applied pharmacology.

[30]  N. Diawara,et al.  Polycyclic aromatic hydrocarbon-induced oxidative stress and lipid peroxidation in relation to immunological alteration , 2010, Occupational and Environmental Medicine.

[31]  S. Caudill,et al.  Concentration and profile of 22 urinary polycyclic aromatic hydrocarbon metabolites in the US population. , 2008, Environmental research.

[32]  Kyu Bong Kim,et al.  Oxidative stress to DNA, protein, and antioxidant enzymes (superoxide dismutase and catalase) in rats treated with benzo(a)pyrene. , 1997, Cancer letters.

[33]  R. Sram,et al.  Seasonal variability of oxidative stress markers in city bus drivers. Part II. Oxidative damage to lipids and proteins. , 2008, Mutation research.

[34]  F. Jongeneelen.  Benchmark guideline for urinary 1-hydroxypyrene as biomarker of occupational exposure to polycyclic aromatic hydrocarbons. , 2001, The Annals of occupational hygiene.

[35]  A. Valavanidis,et al.  Airborne Particulate Matter and Human Health: Toxicological Assessment and Importance of Size and Composition of Particles for Oxidative Damage and Carcinogenic Mechanisms , 2008, Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews.

[36]  J. Turrens.  Superoxide Dismutase and Catalase , 2010 .

[37]  L. Knudsen,et al.  Urinary 1-hydroxypyrene and mutagenicity in bus drivers and mail carriers exposed to urban air pollution in Denmark. , 2004, Mutation research.

[38]  K. Hayakawa,et al.  Simultaneous determination of urinary hydroxylated metabolites of naphthalene, fluorene, phenanthrene, fluoranthene and pyrene as multiple biomarkers of exposure to polycyclic aromatic hydrocarbons , 2006, Analytical and bioanalytical chemistry.

[39]  S. Rappaport,et al.  Environmental and biological monitoring of benzene during self-service automobile refueling. , 2000, Environmental health perspectives.

[40]  J. Angerer,et al.  New reference value and the background exposure for the PAH metabolites 1-hydroxypyrene and 1- and 2-naphthol in urine of the general population in Germany: basis for validation of human biomonitoring data in environmental medicine. , 2008, International journal of hygiene and environmental health.

[41]  Michal Krzyzanowski,et al.  Health Effects of Transport-related Air Pollution , 2005 .

[42]  Bensu Karahalil,et al.  Chromosomal damage in peripheral blood lymphocytes of traffic policemen and taxi drivers exposed to urban air pollution. , 2002, Chemosphere.

[43]  E. Cavalieri,et al.  Central role of radical cations in metabolic activation of polycyclic aromatic hydrocarbons. , 1995, Xenobiotica; the fate of foreign compounds in biological systems.

[44]  J. Cocker,et al.  An assessment of occupational exposure to polycyclic aromatic hydrocarbons in the UK. , 2006, The Annals of occupational hygiene.

[45]  M. Emre,et al.  Effects of benzo(a)pyrene and ethanol on oxidative stress of brain, lung tissues and lung morphology in rats. , 2007, The Chinese journal of physiology.

[46]  W B Jakoby,et al.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. , 1974, The Journal of biological chemistry.

[47]  L. Terada.  Specificity in reactive oxidant signaling: think globally, act locally , 2006, The Journal of Cell Biology.

[48]  K. Kannan,et al.  Benzo[a]pyrene and total polycyclic aromatic hydrocarbons (PAHs) levels in vegetable oils and fats do not reflect the occurrence of the eight genotoxic PAHs , 2010, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.