Entrez Sequences Quick Start

This is a quick start guide for the Entrez Protein, Nucleotide, Expressed Sequence Tag (EST), and Genome Survey Sequence (GSS) databases. The instructions here should allow you to quickly begin searching and using the features of the Entrez sequence databases. • Which of the three databases (nucleotide, EST, or GSS) should I search? • How do I use a simple query, such as a word or a phrase? • How can I make my search more specific with Boolean operators (AND, OR, NOT)? • How do I restrict my search to specific subsets of records such as those from a specific organism, molecule type or source database? • How can I change the format, number, or sorting order of records displayed? • How do I download sequence records to a file on my computer? • How can I change the information that is shown such as optional biological features or sequence? • How do I analyze the sequence data directly or find additional related data? • How can I search for a sub-sequence, or pattern in a protein or nucleotide sequence? • How can I locate and highlight a biological feature in a protein or nucleotide sequence? Which of the three databases containing nucleic acid sequence (Nucleotide, EST, or GSS) should I search?