论文信息 - Positional distribution of ω3 Fatty acids in marine lipid triacylglycerols by high-resolution13C nuclear magnetic resonance spectroscopy

Positional distribution of ω3 Fatty acids in marine lipid triacylglycerols by high-resolution13C nuclear magnetic resonance spectroscopy

The positional distribution [α(1,3)-acyl and ß(2)-acyl] of ω3 fatty acids [18:4(n-3), 20:4(n-3), 20:5(n-3), 22:5(n-3) and 22:6(n-3)] in depot fat of Atlantic salmon (Salmo salar), harp seal oil and cod liver oil triacylglycerols has been examined by13C nuclear magnetic resonance (NMR) spectroscopy. The positional distribution data can be defined from the spectrum of the carbonyl (C1 carbon) and the methylene (C2 and glyceryl carbon) regions. In depot fat of Atlantic salmon and cod liver oil, docosahexaenoic acid (DHA) was concentrated in the ß-position of the triacylglycerides with 72.6 and 74.4%, respectively. Only 3.2% of DHA and 4.6% of eicosapentaenoic acid (EPA) were esterified to the ß-position of the triacylglycerides in harp seal oil. EPA is nearly randomly distributed in cod liver oil and muscle lipids of Atlantic salmon, with 37.8 and 39.7%, respectively, in the ß-position. In general, the13C NMR-derived data were in accordance with corresponding data reported in the literature obtained by conventional techniques.

[1] K. Wollenberg. Quantitative triacylglycerol analysis of whole vegetable seeds by1H and13C magic angle sample spinning NMR spectroscopy , 1991 .

[2] K. Wollenberg. Quantitative high resolution13C nuclear magnetic resonance of the olefinic and carbonyl carbons of edible vegetable oils , 1990 .

[3] F. Gunstone. 13C-NMR spectra of some synthetic glycerol esters alone and as mixtures , 1990 .

[4] A. Kuksis,et al. Stereospecific analysis of menhaden oil triacylglycerols and resolution of complex polyunsaturated diacylglycerols by gas–liquid chromatography on polar capillary columns , 1990 .

[5] F. Gunstone. The 13C-NMR spectra of oils containing γ-linolenic acid , 1990 .

[6] H. Grasdalen,et al. Quantitative high-resolution13C and1H nuclear magnetic resonance of ω3 fatty acids from white muscle of atlantic salmon (Salmo salar) , 1993 .

[7] W. J. Dyer,et al. A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[8] Y. Ando,et al. Stereospecific analysis of fish oil triacyl-sn-glycerols , 1992 .

[9] H. Grasdalen,et al. Interpretation of the 13C-NMR spectra of omega-3 fatty acids and lipid extracted from the white muscle of Atlantic salmon (Salmo salar) , 1992 .