论文信息 - Identification of budding yeast using a fiber-optic imaging bundle

Identification of budding yeast using a fiber-optic imaging bundle

A successful imaging system has been designed and built for yeast pedigree analysis. The system uses a fiber-optic imaging bundle to recognize single yeast cells. Image processing software has been developed to accurately classify the cells as either budding or not budding a daughter cell. This system is intended to replace the body of a microscope for the detection of budding in a microfluidic system.

[1] A D Stroock,et al. An integrated fluorescence detection system in poly(dimethylsiloxane) for microfluidic applications. , 2001, Analytical chemistry.

[2] Y. Sabharwal,et al. Slit-scanning confocal microendoscope for high-resolution in vivo imaging. , 1999, Applied optics.

[3] A. Gmitro,et al. Confocal microscopy through a fiber-optic imaging bundle. , 1993, Optics letters.

[4] Azriel Rosenfeld,et al. Sequential Operations in Digital Picture Processing , 1966, JACM.

[5] David R Walt,et al. Optical imaging fiber-based single live cell arrays: a high-density cell assay platform. , 2002, Analytical chemistry.

[6] Mehdi Hatamian,et al. A real-time two-dimensional moment generating algorithm and its single chip implementation , 1986, IEEE Trans. Acoust. Speech Signal Process..