Real-time PCR fluorescent chemistries.

There are more than a dozen formats available for the fluorescent detection of amplified DNA in kinetic (real-time) PCR. These chemistries are adaptable to most real-time PCR instruments and may offer benefits over the usual manufacturer-recommended chemistries for the instrument. The most popular chemistries are the generic dye, SYBR Green I, TaqMan, and hybridization probes. However, there are now new dyes being reported with superior fluorescent detection and product resolution; as well as new probe formats that may offer advanced multiplexing opportunities for quantification and genotyping.

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