Synthesis and tuberculostatic activity of some (4-phenylpiperazin-1-ylmethyl)-1,3,4-oxadiazole and (4-phenylpiperazin-1-ylmethyl)-1,2,4-triazole derivatives.

The phenylpiperazineacetic hydrazide cyclization product. 5-(4-phenylpiperazin-1-ylmethyl)-1,3,4-oxadiazole-2-thiol (1) upon alkylation gave the S-methyl derivative (2). next it was tranmsformed into 1-phenyl-4-(5-pyrrolidin-1-yl-1,3,4-oxadiazol-2-ylmethyl)-piperazine (3). Upon the reaction of I with 1.2-dibromoethane. the derivative (4) containing two joint 1,3,4-oxadiazole systems was obtained. The reactions of compound 1 with chloroacetonitrile and chloroacetic acid gave the corresponding nitrile and carboxylic acid (5, 6) as well. The 4-amino-1,2,4-triazole-3-thiol derivative (7) was obtained in the reaction of compound I with hydrazine hydrate. The phenylpiperazinacetic acid hydrazide was transformed into 1,3,4-oxadiazol-2-ylamine (8), and this, subsequently, into the 2-ethoxy-1,2,4-triazole derivative (9). The compounds obtained were tested in vitro for their tuberculostatic activity. The minimum inhibiting concentrations (MIC) were within 25 - 100 mg/ml.