Measurements of morphology and refractive indexes on human downy hairs using three-dimensional quantitative phase imaging

Abstract. We present optical measurements of morphology and refractive indexes (RIs) of human downy arm hairs using three-dimensional (3-D) quantitative phase imaging techniques. 3-D RI tomograms and high-resolution two-dimensional synthetic aperture images of individual downy arm hairs were measured using a Mach–Zehnder laser interferometric microscopy equipped with a two-axis galvanometer mirror. From the measured quantitative images, the RIs and morphological parameters of downy hairs were noninvasively quantified including the mean RI, volume, cylinder, and effective radius of individual hairs. In addition, the effects of hydrogen peroxide on individual downy hairs were investigated.

[1]  S. Newton,et al.  Characterization of human scalp hairs by optical low-coherence reflectometry. , 1995, Optics letters.

[2]  P. Corcuff,et al.  3D reconstruction of human hair by confocal microscopy , 1993 .

[3]  P. L. Kirk,et al.  Human Hair Studies: III. Refractive Index of Crown Hair , 1941 .

[4]  YongKeun Park,et al.  Biomedical applications of holographic microspectroscopy [invited]. , 2014, Applied optics.

[5]  YongKeun Park,et al.  High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography , 2013, Journal of biomedical optics.

[6]  YoungJu Jo,et al.  Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications , 2013, Sensors.

[7]  P. E. Wheeler The evolution of bipedality and loss of functional body hair in hominids , 1984 .

[8]  V. Lauer New approach to optical diffraction tomography yielding a vector equation of diffraction tomography and a novel tomographic microscope , 2002, Journal of microscopy.

[9]  Kyoohyun Kim,et al.  Label-free characterization of white blood cells by measuring 3D refractive index maps. , 2015, Biomedical optics express.

[10]  Gabriel Popescu,et al.  Quantitative phase imaging of live cells using fast Fourier phase microscopy. , 2007, Applied optics.

[11]  G. Skopp,et al.  Ultrastructural alterations and environmental exposure influence the opiate concentrations in hair of drug addicts , 2005, International Journal of Legal Medicine.

[12]  Takehito Imai The influence of hair bleach on the ultrastructure of human hair with special reference to hair damage. , 2011, Okajimas folia anatomica Japonica.

[13]  Subra Suresh,et al.  Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient. , 2012, Acta biomaterialia.

[14]  Zhuo Wang,et al.  Jones phase microscopy of transparent and anisotropic samples. , 2008, Optics letters.

[15]  D. Sampson,et al.  High-resolution, wide-field object reconstruction with synthetic aperture Fourier holographic optical microscopy. , 2009, Optics express.

[16]  M. Green,et al.  Human hair growth in vitro. , 1990, Journal of cell science.

[17]  I. Nehls,et al.  Degradation of the ethyl glucuronide content in hair by hydrogen peroxide and a non-destructive assay for oxidative hair treatment using infra-red spectroscopy. , 2014, Forensic science international.

[18]  P. So,et al.  Diffraction optical tomography using a quantitative phase imaging unit. , 2014, Optics letters.

[19]  C. Fang-Yen,et al.  Optical diffraction tomography for high resolution live cell imaging. , 2009, Optics express.

[20]  P. Marquet,et al.  Marker-free phase nanoscopy , 2013, Nature Photonics.

[21]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[22]  Kyoohyun Kim,et al.  Alterations in cell surface area and deformability of individual human red blood cells in stored blood , 2015, 1506.05259.

[23]  S. Comaish,et al.  Scanning electron microscopy of normal and abnormal hair shafts. , 1970, Archives of dermatology.

[24]  YongKeun Park,et al.  Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells , 2009, BiOS.

[25]  YongKeun Park,et al.  Quantitative phase imaging unit. , 2014, Optics letters.

[26]  Bharat Bhushan,et al.  Investigation of scale effects and directionality dependence on friction and adhesion of human hair using AFM and macroscale friction test apparatus. , 2006, Ultramicroscopy.

[27]  D. Sampson,et al.  Synthetic aperture fourier holographic optical microscopy. , 2006, Physical review letters.

[28]  Gabriel Popescu,et al.  Optical imaging of cell mass and growth dynamics. , 2008, American journal of physiology. Cell physiology.

[29]  M. Takeda,et al.  Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry , 1982 .

[30]  Kyoohyun Kim,et al.  Synthetic Fourier transform light scattering. , 2013, Optics express.

[31]  G. Truskey,et al.  Quantitative microscopy and nanoscopy of sickle red blood cells performed by wide field digital interferometry. , 2011, Journal of biomedical optics.

[32]  R. Wepf,et al.  Structural investigations of human hairs by spectrally resolved ellipsometry. , 2006, Journal of biomedical optics.

[33]  R. Happle,et al.  Cytokines and growth factors influence hair growth in vitro. Possible implications for the pathogenesis and treatment of alopecia areata , 1996, Archives of Dermatological Research.

[34]  Gabriel Popescu,et al.  Measurement of red blood cell mechanics during morphological changes , 2010, Proceedings of the National Academy of Sciences.

[35]  L. Wolfram,et al.  The Mechanism of Hair Bleaching , 1970 .

[36]  O. Lunde A study of body hair density and distribution in normal women. , 1984, American journal of physical anthropology.

[37]  Jong Chul Ye,et al.  Real-time Visualization of 3-d Dynamic Microscopic Objects Using Optical Diffraction Tomography References and Links , 2022 .

[38]  Zhuo Wang,et al.  Tissue refractive index as marker of disease. , 2011, Journal of biomedical optics.

[39]  J. Gardner,et al.  Human hair morphology: a scanning electron microscopy study on a male Caucasoid and a computerized classification of regional differences. , 1990, Scanning microscopy.

[40]  J. Baldeschwieler,et al.  Atomic force microscopy of human hair cuticles: a microscopic study of environmental effects on hair morphology. , 1995, The Journal of investigative dermatology.

[41]  A. Diefenbacher,et al.  Fatty acid ethyl esters in hair as markers of alcohol consumption. Segmental hair analysis of alcoholics, social drinkers, and teetotalers. , 2001, Clinical chemistry.

[42]  Yongkeun Park,et al.  Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum , 2008, Proceedings of the National Academy of Sciences.

[43]  Jong Chul Ye,et al.  Comparative study of iterative reconstruction algorithms for missing cone problems in optical diffraction tomography. , 2015, Optics express.

[44]  Zeev Zalevsky,et al.  Synthetic aperture superresolution with multiple off-axis holograms. , 2006, Journal of the Optical Society of America. A, Optics, image science, and vision.

[45]  P. Kintz Analytical and Practical Aspects of Drug Testing in Hair , 2006 .

[46]  J. Strang,et al.  Hair analysis for drugs of abuse , 1990, The Lancet.

[47]  F. Pragst,et al.  State of the art in hair analysis for detection of drug and alcohol abuse. , 2006, Clinica chimica acta; international journal of clinical chemistry.

[48]  J. Strassburger,et al.  Quantitative Fourier transform infrared spectroscopy of , 1985 .

[49]  YongKeun Park,et al.  Three-dimensional refractive index tomograms and deformability of individual human red blood cells from cord blood of newborn infants and maternal blood , 2015, Journal of biomedical optics.

[50]  Kyoohyun Kim,et al.  High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton , 2014 .

[51]  C. Brewer Hair analysis for drugs of abuse , 1990, The Lancet.

[52]  YongKeun Park,et al.  Real-time quantitative phase imaging with a spatial phase-shifting algorithm. , 2011, Optics letters.

[53]  Christian Depeursinge,et al.  Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Cotransporters KCC2 and NKCC1: A Digital Holographic Microscopy Study , 2011, The Journal of Neuroscience.

[54]  Pasquale Memmolo,et al.  3D morphometry of red blood cells by digital holography , 2014, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[55]  F. Leroy,et al.  Current research on ethnic hair. , 2003, Journal of the American Academy of Dermatology.

[56]  C. Hadjur,et al.  Cosmetic assessment of the human hair by confocal microscopy. , 2006, Scanning.

[58]  Jaeduck Jang,et al.  Polarization holographic microscopy for extracting spatio-temporally resolved Jones matrix. , 2012, Optics express.

[59]  B. Forslind,et al.  Human hair form. Morphology revealed by light and scanning electron microscopy and computer aided three-dimensional reconstruction. , 1988, Archives of dermatology.

[60]  P. E. Wheeler The loss of functional body hair in man: the influence of thermal environment, body form and bipedality , 1985 .

[61]  S. P. Gurden,et al.  Quantitative analysis and classification of AFM images of human hair , 2004, Journal of microscopy.

[62]  Nir S. Gov,et al.  Metabolic remodeling of the human red blood cell membrane , 2010, Proceedings of the National Academy of Sciences.

[63]  F. Pragst,et al.  Effect of hair care and hair cosmetics on the concentrations of fatty acid ethyl esters in hair as markers of chronically elevated alcohol consumption. , 2003, Forensic science international.

[64]  Y. Ozaki,et al.  Evaluation of Physical Properties of Human Hair by Diffuse Reflectance Near-Infrared Spectroscopy , 2007, Applied spectroscopy.

[65]  Jaeduck Jang,et al.  Dynamic spectroscopic phase microscopy for quantifying hemoglobin concentration and dynamic membrane fluctuation in red blood cells. , 2012, Optics express.

[66]  W. Sterry,et al.  Variations of hair follicle size and distribution in different body sites. , 2004, The Journal of investigative dermatology.

[67]  Jaeduck Jang,et al.  Spectro-refractometry of individual microscopic objects using swept-source quantitative phase imaging. , 2013, Analytical chemistry.