Analysis of Intraspecific Sequence Variation Among Eight Isolates of the Rumen Symbiont, Isotricha prostoma (Ciliophora), from Two Continents

The internally transcribed spacer regions 1 and 2 (ITS‐1 and ITS‐2) and the 5.8S ribosomal RNA gene of Isotricha prostoma were examined for intraspecific sequence variation. There were no differences in the ITS‐1/5.8S/ITS‐2 region among cattle and sheep isolates of I. prostoma from Australia, Canada, and the United States, indicating that this region is 100% conserved among eight isolates from two continents.

[1]  R. Adlard,et al.  Intraspecific Variation in Cryptocaryon irritans , 1997, The Journal of eukaryotic microbiology.

[2]  D. Lynn,et al.  Phylogeny of the Rumen Ciliates Entodinium, Epidinium and Polyplastron (Litostomatea: Entodiniomorphida) Inferred from Small Subunit Ribosomal RNA Sequences , 1997, The Journal of eukaryotic microbiology.

[3]  F. van Knapen,et al.  Comparison of the internal transcribed spacer, ITS 1, from Toxoplasma gondii isolates and Neospora caninum , 1997, Parasitology Research.

[4]  D. Lynn,et al.  Identifying and Distinguishing Sibling Species in the Tetrahymena pyriformis Complex (Ciliophora, Oligohymenophorea) Using PCR/RFLP Analysis of Nuclear Ribosomal DNA , 1996, The Journal of eukaryotic microbiology.

[5]  P. Klatser,et al.  Mycobacterium leprae isolates from different sources have identical sequences of the spacer region between the 16S and 23S ribosomal RNA genes. , 1994, Microbiology.

[6]  A. von Haeseler,et al.  Comparative evolutionary analysis of rDNA ITS regions in Drosophila. , 1994, Molecular biology and evolution.

[7]  V. Gürtler Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions. , 1993, Journal of general microbiology.

[8]  R. Gasser,et al.  Lack of intraspecific variation in the second internal transcribed spacer (ITS-2) of Trichostrongylus colubriformis ribosomal DNA. , 1993, International journal for parasitology.

[9]  M. Dominguez-Bello,et al.  Microbial Digestive Symbionts of the Crop of the Hoatzin (Opisthocomus hoazin): An Avian Foregut Fermenter , 1993, Physiological Zoology.

[10]  A. R. Smith,et al.  Amplification of rDNA loci to detect and type Neisseria meningitidis and other eubacteria. , 1993, Molecular and cellular probes.

[11]  R. Phillips,et al.  Evolutionary relationships of the salmonid fish genus Salvelinus inferred from DNA sequences of the first internal transcribed spacer (ITS 1) of ribosomal DNA. , 1992, Molecular phylogenetics and evolution.

[12]  B. G. Baldwin Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the compositae. , 1992, Molecular phylogenetics and evolution.

[13]  F. Gannon,et al.  The 16s/23s ribosomal spacer region as a target for DNA probes to identify eubacteria. , 1991, PCR methods and applications.

[14]  M. Allard,et al.  Ribosomal DNA variation within and between species of rodents, with emphasis on the genus Onychomys. , 1991, Molecular biology and evolution.

[15]  E. Myers,et al.  Basic local alignment search tool. , 1990, Journal of molecular biology.

[16]  T. White Analysis of phylogenetic relationships by amplification and direct seaquencing of ribosomal RNA genes , 1990 .

[17]  T. White Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics , 1990 .

[18]  J Engberg,et al.  Complete sequence of the extrachromosomal rDNA molecule from the ciliate Tetrahymena thermophila strain B1868VII. , 1990, Nucleic acids research.

[19]  R. Clarke,et al.  Microbial activity in the forestomach of free-living macropodid marsupials: comparisons with laboratory studies , 1988 .

[20]  G. Olsen,et al.  The small-subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustulata. , 1985, Molecular biology and evolution.

[21]  D. Obendorf The Macropodid Oesophagus IV. Observations on the Protozoan Fauna of the Macropodid Stomach and Oesophagus , 1984 .