Automated liquid-liquid extraction by pneumatic recirculation on a centrifugal microfluidic platform.

In this technical note, a liquid-liquid extraction technique was performed using pneumatic liquid recirculation on a centrifugal microfluidic device. Non-contact pneumatic pumping enabled a multi-cycle liquid-liquid extraction process using aqueous iodine in a potassium iodide solution and hexadecane while requiring a minimal amount of space on the device. The extraction process was completely automated on the device following sample introduction and required only 50 s for each extraction cycle. The pumping rate achieved during liquid recirculation was 120 ± 10 μL/min. A recycling process such as the one demonstrated would be difficult to implement in a conventional centrifugal microfluidic system.

[1]  Eric D Salin,et al.  Pneumatically pumping fluids radially inward on centrifugal microfluidic platforms in motion. , 2010, Analytical chemistry.

[2]  R. Schasfoort,et al.  Recirculation of nanoliter volumes within microfluidic channels. , 2004, Analytical chemistry.

[3]  Eric D Salin,et al.  Magnetically driven solid sample preparation for centrifugal microfluidic devices. , 2009, Analytical chemistry.

[4]  R. Zengerle,et al.  Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. , 2010, Chemical Society reviews.

[5]  Hsiharng Yang,et al.  Centrifugal and Electric Field Forces Dual-Pumping CD-Like Microfluidic Platform for Biomedical Separation , 2004, Biomedical microdevices.

[6]  Eric D Salin,et al.  Pneumatic flow switching on centrifugal microfluidic platforms in motion. , 2011, Analytical chemistry.

[7]  Marc Madou,et al.  Lab on a CD. , 2006, Annual review of biomedical engineering.

[8]  Eric D Salin,et al.  A rapid prototyping technique for valves and filters in centrifugal microfluidic devices. , 2009, Lab on a chip.

[9]  R. Zengerle,et al.  Integrated Sample Preparation, Reaction, and Detection on a High-Frequency Centrifugal Microfluidic Platform , 2005 .

[10]  Tae-Hyeong Kim,et al.  Thermo-pneumatic pumping in centrifugal microfluidic platforms , 2011 .

[11]  R Zengerle,et al.  Batch-mode mixing on centrifugal microfluidic platforms. , 2005, Lab on a chip.

[12]  Roland Zengerle,et al.  Microfluidic platforms for lab-on-a-chip applications. , 2007, Lab on a chip.

[13]  Josiane P Lafleur,et al.  Miniaturised centrifugal solid phase extraction platforms for in-field sampling, pre-concentration and spectrometric detection of organic pollutants in aqueous samples. , 2010, Talanta.

[14]  M. Madou,et al.  Rapid Prototyping of Microfludic Systems , 2007 .

[15]  Eric D Salin,et al.  A valveless pneumatic fluid transfer technique applied to standard additions on a centrifugal microfluidic platform. , 2011, Analytical chemistry.