Hemocytic Differentiation in Hemopoietic Organ of Bombyx mori Larvae

Abstract Bombyx mori L. (Lepidoptera: Bombycidae) larva was investigated with a transmission electron microscopy to determine hemocytic differentiation in the hemopoietic organ located in the prothorax. Three and/or four types of stem cells in compact islets of the organ were observed. Immatured hemocytes in loose islets of the organ were more differentiated and developed than in compact islets. Four types of hemocytes such as prohemocyte, plasmatocyte, granulocyte and oenocytoid were observed in loose islets. Each type of hemocyte was differentiated from each type of stem cell. However, none of spherulocyte was observed. Each type of hemocytes matured in loose islets was discharged into hemolymph by the tearing of acellular membrane covering the islets. These observation strongly suggests that the four kinds of hemocytes except for spherulocytes first appeared in islets and then moved to the region of loose islets in matured form. The more detailed pathway of hemocytic differentiation in B. mori was represented here.

[1]  E. Pimentel Hematopoietic growth factors and cytokines , 1994 .

[2]  H. Varmus,et al.  Cancer genes and hematopoiesis. , 1994, Blood.

[3]  A. Gupta,et al.  Phagocytic recognition in Bombyx mori. , 1991 .

[4]  B. Chang,et al.  Ultrastructure of the Hemopoietic Organ in Euprepocnemis shirakii Bolivar (Orthoptera : Locustidae) , 1990 .

[5]  S. Han,et al.  Arthropod Immune System. II. encapsulation of Implanted Nerve Cord and "Plain Gut" Surgical Suture by Granulocytes of Blattella germanica (L.) (Dictyoptera: Blattellidae) : Immunology , 1989 .

[6]  D. Stoltz,et al.  Observations on cellular immunity and parasitism in the tussock moth , 1987 .

[7]  D. Stoltz,et al.  Apparent haemocytic transformations associated with parasitoid-induced inhibition of immunity in Malacosoma disstria larvae , 1986 .

[8]  L. Bulla,et al.  Structural characterization of the hemocytes of Plodia interpunctella , 1983, Journal of morphology.

[9]  A. Gupta Insect Hemocytes: Hemocyte types: their structures, synonymies, interrelationships, and taxonomic significance , 1979 .

[10]  J. Beaulaton,et al.  Hemocytopoiesis in the oak silkworm Antheraea pernyi and some other lepidoptera. I. Ultrastructural study of normal processes. , 1978, Journal of ultrastructure research.

[11]  C. Hinks,et al.  Haemopoiesis in Lepidoptera. II. The role of the haemopoietic organs , 1977 .

[12]  J. Beaulaton,et al.  Données sur la localisation ultrastructurale d'une activité phénoloxydasique dans les hémocytes circulants d'Antheraea pernyi au dernier âge larvaire , 1977 .

[13]  J. C. Jones The circulatory system of insects , 1977 .

[14]  C. Hinks,et al.  Haemopoiesis in Lepidoptera. I. The multiplication of circulating haemocytes , 1976 .

[15]  J. François Hemocytes et organe hematopoietique de Thermobia domestica (packard) (Thysanura: Lepismatidae) , 1975 .

[16]  J. Lai-Fook,et al.  The structure of the haemocytes of Calpodes ethlius (Lepidoptera) , 1973, Journal of morphology.

[17]  H. Akai,et al.  An ultrastructural study of the haemopoietic organs of the silkworm, Bombyx mori , 1971 .

[18]  J. C. Jones CURRENT CONCEPTS CONCERNING INSECT HEMOCYTES , 1962 .

[19]  William E. Hewson,et al.  The Works of William Hewson, F. R. S., with an Introduction and Notes , 1848, The Medico-chirurgical review.