Electrochemical velocimetry on centrifugal microfluidic platforms.

Expanding upon recent applications of interfacing electricity with centrifugal microfluidic platforms, we introduce electrochemical velocimetry to monitor flow in real-time on rotating fluidic devices. Monitoring flow by electrochemical techniques requires a simple, compact setup of miniaturized electrodes that are embedded within a microfluidic channel and are connected to a peripherally-located potentiostat. On-disc flow rates, determined by electrochemical velocimetry, agreed well with theoretically expected values and with optical measurements. As an application of the presented techniques, the dynamic process of droplet formation and release was recorded, yielding critical information about droplet frequency and volume. Overall, the techniques presented in this work advance the field of centrifugal microfluidics by offering a powerful tool, previously unavailable, to monitor flow in real-time on rotating microfluidic systems.

[1]  Yoon-Kyoung Cho,et al.  One-Step Pathogen Specific DNA Extraction from Whole Blood on a Centrifugal Microfluidic Device , 2007, TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference.

[2]  W. Sansen,et al.  Electrochemical time of flight flow sensor , 2002 .

[3]  S. Wereley,et al.  PIV measurements of a microchannel flow , 1999 .

[4]  Paul C H Li,et al.  Analysis and modeling of flow in rotating spiral microchannels: towards math-aided design of microfluidic systems using centrifugal pumping. , 2011, Lab on a chip.

[5]  N. Djilali,et al.  Integrated electrochemical velocimetry for microfluidic devices , 2006 .

[6]  Joe T. Lin,et al.  Microfabricated Centrifugal Microfluidic Systems: Characterization and Multiple Enzymatic Assays , 1999 .

[7]  David Sinton,et al.  Microscale flow visualization , 2004 .

[8]  Yu Liu,et al.  Modeling of Flow Burst, Flow Timing in Lab‐on‐a‐CD Systems and Its Application in Digital Chemical Analysis , 2008 .

[9]  Roland Zengerle,et al.  The centrifugal microfluidic Bio-Disk platform , 2007 .

[10]  Jintae Kim,et al.  Centrifugal microfluidics for biomedical applications. , 2010, Lab on a chip.

[11]  Roland Zengerle,et al.  Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). , 2010, Lab on a chip.

[12]  Jian Wu,et al.  Micro flow sensor based on two closely spaced amperometric sensors. , 2005, Lab on a chip.

[13]  Mary Amasia,et al.  Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving , 2012 .

[14]  Tae-Hyeong Kim,et al.  Thermo-pneumatic pumping in centrifugal microfluidic platforms , 2011 .

[15]  Yoon‐Kyoung Cho,et al.  Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices. , 2007, Lab on a chip.

[16]  Dong Sung Kim,et al.  Modeling, analysis and design of centrifugal force driven transient filling flow into rectangular microchannel , 2006 .

[17]  Horacio Kido,et al.  Electrolysis-Induced Pneumatic Pressure for Control of Liquids in a Centrifugal System , 2011 .

[18]  D. Beebe,et al.  A particle image velocimetry system for microfluidics , 1998 .

[19]  C L Olson,et al.  Development of a multichannel electrochemical centrifugal analyzer. , 1982, Clinical chemistry.

[20]  Marc J Madou,et al.  The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform. , 2010, Lab on a chip.

[21]  Tae-Hyeong Kim,et al.  Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood. , 2011, Lab on a chip.

[22]  Roland Zengerle,et al.  Centrifugal microfluidic system for primary amplification and secondary real-time PCR. , 2010, Lab on a chip.

[23]  Marc Madou,et al.  Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform. , 2011, Lab on a chip.