A magnetic molecularly imprinted optical chemical sensor for specific recognition of trace quantities of virus

A magnetic resonance light scattering (RLS) sensor based on the molecularly imprinted polymer (MIP) technique was developed for specific recognition of trace quantities of hepatitis A virus (HAV). Through a surface imprinting technique, the virus-magnetic-MIPs (virus-MMIPs) were prepared as the specific identification element, which was based on the effective synthesis of biomimetic polydopamine (PDA) inspired by mussels on the surface of Fe3O4 magnetic nanoparticles. The preparation process of the virus-magnetic-MIPs was simple and rapid under an applied magnetic field. The surface of the magnetic-MIP captured viruses through specific recognition in water, which caused changes to the particle size and shape, and subsequently resulted in changes in the intensity of the RLS. The sensor was applied to determine the amount of HAV in the linear concentration range of 0.02–1.40 nmol L−1, with a low detection limit of 6.2 pmol L−1, and it was successfully applied for the immediate detection of added HAV from a 2000-fold dilution of human serum. More importantly, the proposed strategy addressed the difficulty of virus-MIP detection in the elution process and it is rapid, easy, sensitive, and eco-friendly.

[1]  Chunxia Wu,et al.  Ultrasensitive Detection of Protein Based on Graphene Quantum Dots with Resonance Light Scattering Technique. , 2018, Journal of nanoscience and nanotechnology.

[2]  J. Ge,et al.  Synthesis of Magnetite-Semiconductor-Metal Trimer Nanoparticles through Functional Modular Assembly: A Magnetically Separable Photocatalyst with Photothermic Enhancement for Water Reduction. , 2018, ACS applied materials & interfaces.

[3]  R. Mrówczyński.  Polydopamine-Based Multifunctional (Nano)materials for Cancer Therapy. , 2017, ACS applied materials & interfaces.

[4]  Y. Kitayama,et al.  Post-Cross-Linked Molecular Imprinting with Functional Polymers as a Universal Building Block for Artificial Polymeric Receptors , 2017 .

[5]  Hang Gong,et al.  A virus resonance light scattering sensor based on mussel-inspired molecularly imprinted polymers for high sensitive and high selective detection of Hepatitis A Virus. , 2017, Biosensors & bioelectronics.

[6]  H. Gong,et al.  A virus-MIPs fluorescent sensor based on FRET for highly sensitive detection of JEV. , 2016, Talanta.

[7]  Bin Yang,et al.  Highly selective recognition and fluorescent detection of JEV via virus-imprinted magnetic silicon microspheres , 2016 .

[8]  A. Bosch,et al.  Propidium monoazide RTqPCR assays for the assessment of hepatitis A inactivation and for a better estimation of the health risk of contaminated waters. , 2016, Water research.

[9]  Weihua Hu,et al.  Patterning of Metal Films on Arbitrary Substrates by Using Polydopamine as a UV-Sensitive Catalytic Layer for Electroless Deposition. , 2016, Langmuir : the ACS journal of surfaces and colloids.

[10]  Judith Wackerlig,et al.  Applications of Molecularly Imprinted Polymer Nanoparticles and Their Advances toward Industrial Use: A Review. , 2016, Analytical chemistry.

[11]  Zeynep Altintas,et al.  Comparative investigations for adenovirus recognition and quantification: Plastic or natural antibodies? , 2015, Biosensors & bioelectronics.

[12]  Ping Liu,et al.  Two rhodamine lactam modulated lysosome-targetable fluorescence probes for sensitively and selectively monitoring subcellular organelle pH change. , 2015, Analytica chimica acta.

[13]  Z. Altintas,et al.  Detection of Waterborne Viruses Using High Affinity Molecularly Imprinted Polymers. , 2015, Analytical chemistry.

[14]  Qiang Gao,et al.  Hepatitis A virus and the origins of picornaviruses , 2014, Nature.

[15]  Hongda Li,et al.  Colorimetric and fluorometric dual-modal probes for cyanide detection based on the doubly activated Michael acceptor and their bioimaging applications. , 2014, Analytica chimica acta.

[16]  Y. Zhang,et al.  Fe₃O₄/PVIM-Ni²⁺ magnetic composite microspheres for highly specific separation of histidine-rich proteins. , 2014, ACS applied materials & interfaces.

[17]  Xiaoya Liu,et al.  A facile approach for imprinting protein on the surface of multi-walled carbon nanotubes. , 2014, Talanta.

[18]  Leyu Wang,et al.  Highly selective detection of polycyclic aromatic hydrocarbons using multifunctional magnetic-luminescent molecularly imprinted polymers. , 2013, ACS applied materials & interfaces.

[19]  Guonan Chen,et al.  Facile synthesis of polydopamine-coated molecularly imprinted silica nanoparticles for protein recognition and separation. , 2013, Biosensors & bioelectronics.

[20]  Jian Ji,et al.  Mussel-inspired polydopamine: a biocompatible and ultrastable coating for nanoparticles in vivo. , 2013, ACS nano.

[21]  B. Sellergren,et al.  Molecularly imprinted polydopamine nano-layer on the pore surface of porous particles for protein capture in HPLC column. , 2013, Journal of colloid and interface science.

[22]  M. Nurunnabi,et al.  Surface coating of graphene quantum dots using mussel-inspired polydopamine for biomedical optical imaging. , 2013, ACS applied materials & interfaces.

[23]  S. Vasudevan,et al.  Cyclodextrin-functionalized Fe3O4@TiO2: reusable, magnetic nanoparticles for photocatalytic degradation of endocrine-disrupting chemicals in water supplies. , 2013, ACS nano.

[24]  B. Lorber,et al.  A synthetic nanomaterial for virus recognition produced by surface imprinting , 2013, Nature Communications.

[25]  Thomas Wriedt,et al.  The Mie Theory , 2012 .

[26]  Thomas Wriedt,et al.  The Mie theory : basics and applications , 2012 .

[27]  Z. Chen,et al.  A novel curcumin assay with the metal ion Cu (II) as a simple probe by resonance light scattering technique. , 2009, Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.

[28]  Chenjie Xu,et al.  Ultrasmall c(RGDyK)-coated Fe3O4 nanoparticles and their specific targeting to integrin alpha(v)beta3-rich tumor cells. , 2008, Journal of the American Chemical Society.

[29]  Jian Ling,et al.  Magnetic particle-based sandwich sensor with DNA-modified carbon nanotubes as recognition elements for detection of DNA hybridization. , 2008, Analytical chemistry.

[30]  Haeshin Lee,et al.  Mussel-Inspired Surface Chemistry for Multifunctional Coatings , 2007, Science.

[31]  Chaoqing Dong,et al.  Single‐Molecule Technology for Rapid Detection of DNA Hybridization Based on Resonance Light Scattering of Gold Nanoparticles , 2007, Chembiochem : a European journal of chemical biology.

[32]  Franz L Dickert,et al.  Bioimprinted QCM sensors for virus detection—screening of plant sap , 2004, Analytical and Bioanalytical Chemistry.

[33]  P. Collings,et al.  Resonance light scattering: a new technique for studying chromophore aggregation , 1995, Science.

[34]  C. Bustamante,et al.  Porphyrin Assemblies On DNA As Studied By A Resonance Light-Scattering Technique , 1993 .

[35]  F. Deinhardt.  Prevention of viral hepatitis A: past, present and future. , 1992, Vaccine.