Previous studies have reported that intra-urban variability of NO2 concentrations is even higher than inter-urban variability. In recent years, an increasing number of studies have developed satellite-derived land use regression (LUR) models to predict ground-level NO2 concentrations, though only a few have been conducted at a city scale. In this study, we developed a satellite-derived LUR model to predict seasonal NO2 concentrations at a city scale by including satellite-retrieved NO2 tropospheric column density, population density, traffic indicators, and NOx emission data. The R2 of model fitting and 10-fold cross validation were 0.70 and 0.61 for the satellite-derived seasonal LUR model, respectively. The satellite-based LUR model captured seasonal patterns and fine gradients of NO2 variations at a 100 m × 100 m resolution and demonstrated that NO2 pollution in winter is 1.46 times higher than that in summer. NO2 concentrations declined significantly with increasing distance from roads and with increasing distance from the city center. In Suzhou, 84% of the total population lived in areas with NO2 concentrations exceeding the annual-mean standard at 40 μg/m3 in 2014. This study demonstrated that satellite-retrieved data could help increase the accuracy and temporal resolution of the traditional LUR models at a city scale. This application could support exposure assessment at a high resolution for future epidemiological studies and policy development pertaining to air quality control.