Mechanical pressure and stretch release endothelin-1 from human atherosclerotic coronary arteries in vivo.

BACKGROUND Endothelin-1 (ET-1) is an endothelium-derived vasoactive peptide with mitogenic properties. In vitro, vascular release of ET-1 is increased in response to mechanical stress. The goal of the present study was to examine whether ET-1 is released from human atherosclerotic coronary arteries in vivo in response to mechanical pressure and stretch and to characterize immunoreactivity for ET-1 and its precursor, big ET-1, within the atheromatous plaque. METHODS AND RESULTS Circulating ET-1 levels were measured in 20 patients before and after coronary angioplasty for stable angina at three sampling sites: the femoral artery and the coronary artery segments proximal and distal to the lesion dilated. In addition, atheromatous tissue obtained from 20 patients undergoing directional coronary atherectomy for stable angina were analyzed for immunoreactivity for ET-1 and big ET-1. In patients undergoing angioplasty, ET-1 levels in the distal coronary artery increased after balloon dilatation (8.4 +/- 0.9 to 16.4 +/- 2 pg/mL, P < .05); proximal coronary artery and systemic ET-1 levels were unchanged. The degree of mechanical stress applied (product of duration and pressure of balloon inflation) correlated with the change in distal coronary artery ET-1 levels (r = .71, P < .01). Immunoreactivity for big ET-1 and ET-1 was ubiquitous in the extracellular space and the intracellular compartment (macrophages, myointimal cells, myofibroblasts, and endothelial cells) of human coronary atheromatous tissue. CONCLUSIONS Big ET-1 and ET-1 immunoreactivity is ubiquitous within the intracellular and extracellular compartments of coronary atherosclerotic tissue. ET-1 is released from these sites in response to mechanical stress. These findings support a role for endothelins in the evolution and progression of coronary atherosclerosis in humans.

[1]  A. Lerman,et al.  Endothelin at pathophysiological concentrations mediates coronary vasoconstriction via the endothelin-A receptor. , 1995, Circulation.

[2]  A. Lerman,et al.  The atherogenic potential of endothelin. , 1995, Coronary artery disease.

[3]  S. Douglas,et al.  Selective ETA receptor antagonism with BQ-123 is insufficient to inhibit angioplasty induced neointima formation in the rat. , 1995, Cardiovascular research.

[4]  A. Franco‐Cereceda,et al.  Influence of percutaneous transluminal coronary angioplasty on cardiac release of endothelin, neuropeptide Y and noradrenaline. , 1995, International journal of cardiology.

[5]  T. Saruta,et al.  Pressure enhances endothelin-1 release from cultured human endothelial cells. , 1995, Hypertension.

[6]  A. Zeiher,et al.  Tissue endothelin-1 immunoreactivity in the active coronary atherosclerotic plaque. A clue to the mechanism of increased vasoreactivity of the culprit lesion in unstable angina. , 1995, Circulation.

[7]  A. Davenport,et al.  Secretion of endothelin‐1 and endothelin‐3 by human cultured vascular smooth muscle cells , 1995, British journal of pharmacology.

[8]  S. Manuck,et al.  Antiatherogenic effects of beta-adrenergic blocking agents: theoretical, experimental, and epidemiologic considerations. , 1994, American heart journal.

[9]  G. Montalescot,et al.  Endothelin-1 in patients with coronary heart disease undergoing cardiac catheterization. , 1994, Journal of the American College of Cardiology.

[10]  G. Bondjers.  Anti-atherosclerotic effects of beta-blockers. , 1994, European heart journal.

[11]  J. Marco,et al.  Coronary Vasoconstriction After Percutaneous Transluminal Coronary Angioplast Is Attenuated by Antiadrenergic Agents , 1994, Circulation.

[12]  J. D. Elliott,et al.  A role for endogenous endothelin-1 in neointimal formation after rat carotid artery balloon angioplasty. Protective effects of the novel nonpeptide endothelin receptor antagonist SB 209670. , 1994, Circulation research.

[13]  G. McClellan,et al.  Endothelial cell storage and release of endothelin as a cardioregulatory mechanism. , 1994, Circulation research.

[14]  J. Vane,et al.  Endothelin-1 release from endothelial cells in culture is elevated both acutely and chronically by short periods of mechanical stretch. , 1994, Biochemical and biophysical research communications.

[15]  R. Andersson,et al.  Angiotensin converting enzyme inhibitors and atherosclerosis. , 1994, Journal of physiology and pharmacology : an official journal of the Polish Physiological Society.

[16]  H. Kawasaki,et al.  Inhibition of plasma endothelin-1 concentration by captopril in patients with essential hypertension. , 1994, Clinical nephrology.

[17]  M. Condorelli,et al.  Local effect of serotonin released during coronary angioplasty. , 1994, The New England journal of medicine.

[18]  S. Kaul,et al.  Effect of percutaneous transluminal coronary angioplasty on circulating endothelin levels. , 1993, The American journal of cardiology.

[19]  V. Fuster,et al.  Circulating and Tissue Endothelin Immunoreactivit in Hypercholesterolemic Pigs , 1993, Circulation.

[20]  L. Malatino,et al.  Release of immunoreactive endothelin from the heart during percutaneous transluminal coronary angioplasty. , 1993, American heart journal.

[21]  D. Crossman,et al.  Expression of basic and acidic fibroblast growth factors and their receptor in normal and atherosclerotic human arteries. , 1993, Cardiovascular Research.

[22]  T. Ogihara,et al.  Captopril inhibits endothelin-1 secretion from endothelial cells through bradykinin. , 1993, Hypertension.

[23]  P. Davies,et al.  Mechanical stress mechanisms and the cell. An endothelial paradigm. , 1993, Circulation research.

[24]  M. Kuchan,et al.  Shear stress regulates endothelin-1 release via protein kinase C and cGMP in cultured endothelial cells. , 1993, The American journal of physiology.

[25]  S. Douglas,et al.  Endothelin-1 promotes neointima formation after balloon angioplasty in the rat. , 1993, Journal of cardiovascular pharmacology.

[26]  F. Crea,et al.  Myocardial ischemia caused by distal coronary vasoconstriction. , 1992, The American journal of cardiology.

[27]  R. Kirsner,et al.  The effects of endothelin-1 on human dermal fibroblast growth and synthetic activity. , 1992, The Journal of surgical research.

[28]  M. Nakamura,et al.  Inhibition by angiotensin converting enzyme inhibitors of endothelin secretion from cultured human endothelial cells. , 1992, Life sciences.

[29]  Antony Sj,et al.  Endothelin-1 secretion by human fibroblasts in culture: effects of cell density and IFN-beta. , 1991 .

[30]  M. Kohno,et al.  Circulating immunoreactive endothelin in patients undergoing percutaneous transluminal coronary angioplasty. , 1991, Metabolism: clinical and experimental.

[31]  J. Fruchart,et al.  Modified low density lipoproteins activate human macrophages to secrete immunoreactive endothelin , 1991, FEBS letters.

[32]  A. Lerman,et al.  Circulating and tissue endothelin immunoreactivity in advanced atherosclerosis. , 1991, The New England journal of medicine.

[33]  F. Crea,et al.  Abnormal Vasomotor Changes Early After Coronary Angioplasty: A Quantitative Arteriographic Study of Their Time Course , 1991, Circulation.

[34]  W. Edwards,et al.  Differential histopathology of primary atherosclerotic and restenotic lesions in coronary arteries and saphenous vein bypass grafts: analysis of tissue obtained from 73 patients by directional atherectomy. , 1991, Journal of the American College of Cardiology.

[35]  K. Takakura,et al.  The role of cerebral microvessel endothelium in regulation of cerebral blood flow through production of endothelin-1. , 1991, Journal of cardiovascular pharmacology.

[36]  K. Margulies,et al.  Endothelin: a new cardiovascular regulatory peptide. , 1990, Mayo Clinic proceedings.

[37]  D. Heistad,et al.  Vascular responses to endothelin-1 in atherosclerotic primates. , 1990, Arteriosclerosis.

[38]  D. Holmes,et al.  Coronary atherectomy: first 50 patients at the Mayo Clinic. , 1989, Mayo Clinic proceedings.

[39]  C. White,et al.  INTENSE MICROVASCULAR CONSTRICTION AFTER ANGIOPLASTY OF ACUTE THROMBOTIC CORONARY ARTERIAL LESIONS , 1989, The Lancet.

[40]  A. Maseri,et al.  Endothelin‐1 Is a Potent Constrictor in Conductive and Resistive Coronary Arteries , 1989, Journal of cardiovascular pharmacology.

[41]  D. Ku,et al.  Hemodynamics and atherosclerosis. Insights and perspectives gained from studies of human arteries. , 1988, Archives of pathology & laboratory medicine.

[42]  R. Ross.  The pathogenesis of atherosclerosis--an update. , 1986, The New England journal of medicine.

[43]  L. Hillis,et al.  Percutaneous transluminal coronary angioplasty. , 1994, The American journal of the medical sciences.

[44]  M. Gimbrone,et al.  Influence of hemodynamic forces on vascular endothelial function. In vitro studies of shear stress and pinocytosis in bovine aortic cells. , 1984, The Journal of clinical investigation.

[45]  Stehbens We,et al.  Hemodynamics and atherosclerosis. , 1982, Biorheology.