High numerical aperture vectorial imaging in coherent optical microscopes.

Imaging systems are typically partitioned into three components: focusing of incident light, scattering of incident light by an object and imaging of scattered light. We present a model of high Numerical Aperture (NA) imaging systems which differs from prior models as it treats each of the three components of the imaging system rigorously. It is well known that when high NA lenses are used the imaging system must be treated with vectorial analysis. This in turn requires that the scattering of light by the object be calculated rigorously according to Maxwell's equations. Maxwell's equations are solvable analytically for only a small class of scattering objects necessitating the use of rigorous numerical methods for the general case. Finally, rigorous vectorial diffraction theory and focusing theory are combined to calculate the image of the scattered light. We demonstrate the usefulness of the model through examples.

[1]  C. Sheppard,et al.  The image of a single point in microscopes of large numerical aperture , 1982, Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences.

[2]  J. Goodman Introduction to Fourier optics , 1969 .

[3]  A. Bayliss,et al.  Radiation boundary conditions for wave-like equations , 1980 .

[4]  T. Gaylord,et al.  Rigorous coupled-wave analysis of planar-grating diffraction , 1981 .

[5]  P. Török,et al.  Calculation of the image of an arbitrary vectorial electromagnetic field. , 2007, Optics express.

[6]  Jean-Pierre Berenger,et al.  A perfectly matched layer for the absorption of electromagnetic waves , 1994 .

[7]  P. Török,et al.  Propagation of electromagnetic dipole waves through dielectric interfaces. , 2000, Optics letters.

[8]  P. Török,et al.  Rigorous analysis of spheres in Gauss-Laguerre beams. , 2007, Optics express.

[9]  Optimising the image contrast of conventional and confocal optical microscopes imaging finite sized spherical gold scatterers , 1998 .

[10]  Peter Török,et al.  Vectorial, high numerical aperture study of Nomarski's differential interference contrast microscope. , 2005, Optics express.

[11]  A. Majda,et al.  Absorbing boundary conditions for the numerical simulation of waves , 1977 .

[12]  P. Varga,et al.  Electromagnetic diffraction of light focused through a stratified medium. , 1997, Applied optics.

[13]  F. Tangherlini,et al.  Optical Constants of Silver, Gold, Copper, and Aluminum. II. The Index of Refraction n , 1954 .

[14]  G. Arfken Mathematical Methods for Physicists , 1967 .

[15]  J. Judkins,et al.  Finite-difference time-domain modeling of nonperfectly conducting metallic thin-film gratings , 1995 .

[16]  Analysis of the polarization-dependent diffraction from a metallic grating by use of a three-dimensional combined vectorial method. , 2004, Journal of the Optical Society of America. A, Optics, image science, and vision.

[17]  E. Wolf,et al.  Electromagnetic diffraction in optical systems, II. Structure of the image field in an aplanatic system , 1959, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[18]  Peter Török,et al.  Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integral representation , 1995 .

[19]  Olivier J. F. Martin,et al.  Iterative scheme for computing exactly the total field propagating in dielectric structures of arbitrary shape , 1994 .

[20]  R. W. Christy,et al.  Optical Constants of the Noble Metals , 1972 .

[21]  Peter R T Munro,et al.  Rigorous near- to far-field transformation for vectorial diffraction calculations and its numerical implementation. , 2006, Journal of the Optical Society of America. A, Optics, image science, and vision.

[22]  Christian Hafner,et al.  Multiple multipole method with automatic multipole setting applied to the simulation of surface plasmons in metallic nanostructures. , 2002, Journal of the Optical Society of America. A, Optics, image science, and vision.

[23]  R. B. Standler,et al.  A frequency-dependent finite-difference time-domain formulation for dispersive materials , 1990 .

[24]  P. Munro,et al.  Vectorial, high-numerical-aperture study of phase-contrast microscopes. , 2004, Journal of the Optical Society of America. A, Optics, image science, and vision.

[25]  Silvania F. Pereira,et al.  Numerical analysis of a slit-groove diffraction problem , 2007 .

[26]  G. Mur Absorbing Boundary Conditions for the Finite-Difference Approximation of the Time-Domain Electromagnetic-Field Equations , 1981, IEEE Transactions on Electromagnetic Compatibility.

[27]  K. Yee Numerical solution of initial boundary value problems involving maxwell's equations in isotropic media , 1966 .

[28]  Tony Wilson,et al.  Theory for confocal and conventional microscopes imaging small dielectric scatterers , 1998 .