Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis.

In this study, we investigated a novel application of matrix solid-phase dispersion (MSPD) methodology for the extraction of endogenous peptides from porcine hypothalamus tissue samples. Several experimental factors of the MSPD procedure were examined. Finally, silica-based octadecyl was chosen as dispersing material and blended with 0.25 g porcine hypothalamus at a ratio of 5, and 10 mL of 60% acetonitrile with 0.2% formic acid in water was chosen as the extraction and elution solvent. This MSPD extraction method was compared to the classic acid extraction method. More peaks were observed in the MSPD extracts (74±5) by MALDI-TOF MS than in acid extracts (34±5). Moreover, 14 potential endogenous peptides were identified in the MSPD extracts after nanoLC-MS/MS analysis, while only 2 endogenous peptides in the acid extracts. These results indicated that MSPD could be employed as a simple and efficient method for the extraction of endogenous peptides from tissues.

[1]  S. Mohammed,et al.  Improved identification of endogenous peptides from murine nervous tissue by multiplexed peptide extraction methods and multiplexed mass spectrometric analysis. , 2009, Journal of proteome research.

[2]  Martha U. Gillette,et al.  Neuropeptidomics of the Supraoptic Rat Nucleus , 2008, Journal of proteome research.

[3]  Piero Giansanti,et al.  Recent developments in matrix solid-phase dispersion extraction. , 2010, Journal of chromatography. A.

[4]  S. Barker,et al.  Matrix solid-phase dispersion. , 2000, Journal of chromatography. A.

[5]  M. Eddleston,et al.  Simultaneous quantification of the organophosphorus pesticides dimethoate and omethoate in porcine plasma and urine by LC-ESI-MS/MS and flow-injection-ESI-MS/MS. , 2010, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[6]  B. Shao,et al.  Analysis of eight free progestogens in eggs by matrix solid-phase dispersion extraction and very high pressure liquid chromatography with tandem mass spectrometry. , 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[7]  Lloyd D. Fricker,et al.  Quantitative Neuropeptidomics of Microwave-irradiated Mouse Brain and Pituitary* , 2005, Molecular & Cellular Proteomics.

[8]  P. Svenningsson,et al.  Peptidomics-based discovery of novel neuropeptides. , 2003, Journal of proteome research.

[9]  Hua Wei,et al.  Optimization of the matrix solid phase dispersion extraction procedure for the analysis of polybrominated diphenyl ethers in human placenta. , 2009, Analytical chemistry.

[10]  S. Barker.  Matrix solid phase dispersion (MSPD). , 2007, Journal of biochemical and biophysical methods.

[11]  H. Mu,et al.  Multiresidue determination of fluoroquinolones, organophosphorus and N-methyl carbamates simultaneously in porcine tissue using MSPD and HPLC-DAD. , 2009, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[12]  Bing Shao,et al.  Analysis of alkylphenol and bisphenol A in eggs and milk by matrix solid phase dispersion extraction and liquid chromatography with tandem mass spectrometry. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[13]  Lingjun Li,et al.  Rat neuropeptidomics by LC-MS/MS and MALDI-FTMS: Enhanced dissection and extraction techniques coupled with 2D RP-RP HPLC. , 2006, Journal of proteome research.

[14]  M. Schrader,et al.  Peptidomics: the comprehensive analysis of peptides in complex biological mixtures. , 2012, Combinatorial chemistry & high throughput screening.

[15]  L. Berrueta,et al.  Matrix solid-phase dispersion technique for the determination of a new antiallergic drug, bilastine, in rat faeces. , 2001, Journal of chromatography. B, Biomedical sciences and applications.

[16]  F. Strand.  Neuropeptides: Regulators of Physiological Processes , 1998 .

[17]  M. Baker,et al.  Evaluation of endogenous plasma peptide extraction methods for mass spectrometric biomarker discovery. , 2007, Journal of proteome research.

[18]  Fa-Yun Che,et al.  Optimization of neuropeptide extraction from the mouse hypothalamus. , 2007, Journal of proteome research.

[19]  B. Buszewski,et al.  Optimization of a matrix solid-phase dispersion method for the determination analysis of carbendazim residue in plant material. , 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.