Feasibility studies on the fermentative hydrogen production by recombinant Escherichia coli BL-21

Abstract Fermentative hydrogen production has been carried out both in batch and continuous system using recombinant Escherichia coli BL 21. Effect of various process parameters, viz. size of inoculum, initial medium pH, initial substrate concentration, temperature and dilution rate, was examined with respect to maximum hydrogen productivity. Maximum hydrogen production rate obtained in this study was 66 mmol/(l h) using glucose as substrate at a dilution rate of 0.55 h −1 in the immobilized whole cell bioreactor. Black strap molasses, a by-product of sugar industry, was found suitable as a feedstock for fermentation using which maximum rate of hydrogen production was 97.4 mmol/(l h). Energy analysis reveals that the percentage of gaseous energy recovery obtained in this study using recombinant E. coli BL 21 and considering glucose as a substrate was 26.68%. A comparison of hydrogen production characteristics between the wild strain Enterobacter cloacae IIT-BT-08 and that of the recombinant E. coli BL21 indicates that optimum initial pH, initial glucose concentration and reaction temperature are almost comparable and/or marginally same for both the strains. However, the yield of hydrogen (mol H 2 /(mol glucose)) with the recombinant strain was 3.12 mol H 2 /(mol glucose) which was much higher than that reported for the wild strain.

[1]  M. Ladisch,et al.  Optimum ph and temperature conditions for xylose fermentation by Pichia stipitis , 1990, Biotechnology and bioengineering.

[2]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[3]  You-Kwan Oh,et al.  Fermentative biohydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19 , 2003 .

[4]  Shigeharu Tanisho,et al.  Fermentative hydrogen evolution by Enterobacter aerogenes strain E.82005 , 1987 .

[5]  Debabrata Das,et al.  Improvement of fermentative hydrogen production: various approaches , 2004, Applied Microbiology and Biotechnology.

[6]  Shigeharu Tanisho,et al.  Continuous hydrogen production from molasses by fermentation using urethane foam as a support of flocks , 1995 .

[7]  Peter Lindblad,et al.  Realizing the hydrogen future: the International Energy Agency's efforts to advance hydrogen energy technologies , 2003 .

[8]  L. K. Bowles,et al.  Effects of butanol on Clostridium acetobutylicum , 1985, Applied and environmental microbiology.

[9]  Debabrata Das,et al.  Isolation and molecular characterization of hydrogenase gene from a high rate of hydrogen-producing bacterial strain Enterobacter cloacae IIT-BT 08 , 2002 .

[10]  T. Ghose,et al.  Rapid ethanol fermentation in immobilized yeast cell reactor , 1980 .

[11]  Sang-Eun Oh,et al.  Biological hydrogen production measured in batch anaerobic respirometers. , 2002, Environmental science & technology.

[12]  S. Tanisho,et al.  Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenes , 1994 .

[13]  Debabrata Das,et al.  Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. , 2001 .

[14]  C-C. Chen,et al.  Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as the limiting substrate , 2001, Applied Microbiology and Biotechnology.

[15]  D. L. Hawkes,et al.  Sustainable fermentative hydrogen production: challenges for process optimisation , 2002 .

[16]  Jun Hirose,et al.  Characteristics of hydrogen production by aciduric Enterobacter aerogenes strain HO-39 , 1995 .

[17]  J. Lay,et al.  Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose. , 2001, Biotechnology and bioengineering.

[18]  R. Nandi,et al.  Microbial production of hydrogen: an overview. , 1998, Critical reviews in microbiology.

[19]  Eun Yeol Lee,et al.  Fermentative hydrogen production by a new chemoheterotrophic bacterium Rhodopseudomonas palustris P4 , 2002 .

[20]  Debabrata Das,et al.  Enhancement of hydrogen production by Enterobacter cloacae IIT-BT 08. , 2000 .

[21]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[22]  C. Lin,et al.  Biohydrogen production by mesophilic fermentation of food wastewater. , 2004, Water science and technology : a journal of the International Association on Water Pollution Research.

[23]  Bruno Fabiano,et al.  Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes , 2002 .

[24]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[25]  Tong Zhang,et al.  Biohydrogen production from starch in wastewater under thermophilic condition. , 2003, Journal of environmental management.

[26]  D. Das,et al.  Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08. , 2004, Biochemical and biophysical research communications.

[27]  N. Kosaric,et al.  Microbial production of hydrogen , 1978 .