论文信息 - Calcium and voltage imaging in arrhythmia models by high-speed microscopy

Calcium and voltage imaging in arrhythmia models by high-speed microscopy

Alterations in intracellular cardiomyocyte calcium handling have a key role in initiating and sustaining arrhythmias. Arrhythmogenic calcium leak from sarcoplasmic reticulum (SR) can be attributed to all means by which calcium exits the SR store in an abnormal fashion. Abnormal SR calcium exit maymanifest as intracellular Ca2+ sparks and/or Ca2+ waves. Ca2+ signaling in arrhythmogenesis has been mainly studied in isolated cardiomyocytes and given that the extracellular matrix influences both Ca2+ and membrane potential dynamics in the intact heart and underlies environmentally mediated changes, understanding how Ca2+ and voltage are regulated in the intact heart will represent a tremendous advancement in the understanding of arrhythmogenic mechanisms. Using novel high-speed multiphoton microscopy techinques, such as multispot and random access, we investigated animal models with inherited and acquired arrhythmias to assess the role of Ca2+ and voltage signals as arrhythmia triggers in cell and subcellular components of the intact heart and correlate these with electrophysiology.

[1] Sergio Fantini,et al. Multifocal multiphoton microscopy based on multianode photomultiplier tubes. , 2007, Optics express.

[2] P. Saggau,et al. High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators. , 1997, Biophysical journal.

[3] P. So,et al. Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes. , 1997, Optics letters.

[4] M R Taghizadeh,et al. Multiphoton multifocal microscopy exploiting a diffractive optical element. , 2003, Optics letters.

[5] Leonardo Sacconi,et al. Optical recording of electrical activity in intact neuronal networks with random access second-harmonic generation microscopy. , 2008, Optics express.