Surface chemistries for antibody microarrays.

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.

[1]  G. Whitesides,et al.  Formation of self-assembled monolayers by chemisorption of derivatives of oligo(ethylene glycol) of structure HS(CH2)11(OCH2CH2)mOH on gold , 1991 .

[2]  M. Smyth,et al.  Oriented immobilization of antibodies and its applications in immunoassays and immunosensors. , 1996, The Analyst.

[3]  F. Ligler,et al.  Effectiveness of protein A for antibody immobilization for a fiber optic biosensor. , 1997, Biosensors & bioelectronics.

[4]  K. Schauenstein,et al.  Interheavy disulfide bridge in immunoglobulin G (IgG) reacting with dithionitrobenzoate. A unique feature in serum proteins. , 1997, Journal of biochemical and biophysical methods.

[5]  A. Mirzabekov,et al.  Manual manufacturing of oligonucleotide, DNA, and protein microchips. , 1997, Analytical biochemistry.

[6]  R. Ekins,et al.  Ligand assays: from electrophoresis to miniaturized microarrays. , 1998, Clinical chemistry.

[7]  S. Wölfl,et al.  Preparation of DNA and protein micro arrays on glass slides coated with an agarose film. , 2000, Nucleic acids research.

[8]  B. Stillman,et al.  FAST slides: a novel surface for microarrays. , 2000, BioTechniques.

[9]  S. Schreiber,et al.  Printing proteins as microarrays for high-throughput function determination. , 2000, Science.

[10]  P. Brown,et al.  Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions , 2001, Genome Biology.

[11]  R. Huang,et al.  Detection of multiple proteins in an antibody-based protein microarray system. , 2001, Journal of immunological methods.

[12]  C. Niemeyer,et al.  Dendrimer‐Activated Solid Supports for Nucleic Acid and Protein Microarrays , 2001, Chembiochem : a European journal of chemical biology.

[13]  D. Leckband,et al.  A quantitative assessment of heterogeneity for surface-immobilized proteins. , 2001, Analytical chemistry.

[14]  S. Nock,et al.  Monolayers of derivatized poly(L-lysine)-grafted poly(ethylene glycol) on metal oxides as a class of biomolecular interfaces. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[15]  M. Gerstein,et al.  Global Analysis of Protein Activities Using Proteome Chips , 2001, Science.

[16]  H. Lehrach,et al.  Toward optimized antibody microarrays: a comparison of current microarray support materials. , 2002, Analytical biochemistry.

[17]  Qing Zhu,et al.  Intein-mediated biotinylation of proteins and its application in a protein microarray. , 2002, Journal of the American Chemical Society.

[18]  Frances S Ligler,et al.  A microarray immunoassay for simultaneous detection of proteins and bacteria. , 2002, Analytical chemistry.

[19]  Barry Schweitzer,et al.  Measuring proteins on microarrays. , 2002, Current opinion in biotechnology.

[20]  Wei Zhang,et al.  Free Sulfhydryl in Recombinant Monoclonal Antibodies , 2002, Biotechnology progress.

[21]  H. Lehrach,et al.  Next generation of protein microarray support materials: evaluation for protein and antibody microarray applications. , 2003, Journal of chromatography. A.

[22]  Kendall Powell.  Proteomics delivers on promise of cancer biomarkers , 2003, Nature Medicine.

[23]  M. Verma,et al.  New cancer biomarkers deriving from NCI early detection research. , 2003, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[24]  J. Hoheisel,et al.  Antibody microarrays: An evaluation of production parameters , 2003, Proteomics.

[25]  J. Hoheisel,et al.  Solid supports for microarray immunoassays , 2003, Journal of molecular recognition : JMR.

[26]  R. Oyama,et al.  In vitro protein microarrays for detecting protein‐protein interactions: Application of a new method for fluorescence labeling of proteins , 2003, Proteomics.

[27]  F. Höök,et al.  Patterns of DNA‐Labeled and scFv‐Antibody‐Carrying Lipid Vesicles Directed by Material‐Specific Immobilization of DNA and Supported Lipid Bilayer Formation on an Au/SiO2 Template , 2003, Chembiochem : a European journal of chemical biology.

[28]  S. Ekström,et al.  Macro-/nanoporous silicon as a support for high-performance protein microarrays. , 2003, Analytical chemistry.

[29]  B. Haab.  Methods and applications of antibody microarrays in cancer research , 2003, Proteomics.

[30]  Bong Jin Hong,et al.  Self-Assembly of a Dendron through Multiple Ionic Interaction to Give Mesospacing between Reactive Amine Groups on the Surface , 2003 .

[31]  James R. McElhanon,et al.  Dendrimer-activated surfaces for high density and high activity protein chip applications. , 2004, Langmuir : the ACS journal of surfaces and colloids.

[32]  H. Hug,et al.  Advances in recombinant antibody microarrays. , 2004, Clinica chimica acta; international journal of clinical chemistry.

[33]  E. Goldman,et al.  Detection of proteins cross-linked within galactoside polyacrylate-based hydrogels by means of a quantum dot fluororeagent , 2004, Analytical and bioanalytical chemistry.

[34]  Marina Cretich,et al.  A new polymeric coating for protein microarrays. , 2004, Analytical biochemistry.

[35]  Ulrik B Nielsen,et al.  Multiplexed sandwich assays in microarray format. , 2004, Journal of immunological methods.

[36]  Bong Jin Hong,et al.  DNA microarrays on a dendron-modified surface improve significantly the detection of single nucleotide variations in the p53 gene , 2005, Nucleic acids research.

[37]  Bong Jin Hong,et al.  Nanoscale-controlled spacing provides DNA microarrays with the SNP discrimination efficiency in solution phase. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[38]  S. McClintock,et al.  Comparison of antibody array substrates and the use of glycerol to normalize spot morphology. , 2005, Experimental and molecular pathology.

[39]  Johan Ingvarsson,et al.  Microarrays based on affinity‐tagged single‐chain Fv antibodies: Sensitive detection of analyte in complex proteomes , 2005, Proteomics.

[40]  Gilbert S Omenn,et al.  Immunoassay and antibody microarray analysis of the HUPO Plasma Proteome Project reference specimens: Systematic variation between sample types and calibration of mass spectrometry data , 2005, Proteomics.

[41]  H. Lilja,et al.  Macro/nano-structured silicon as solid support for antibody arrays: Surface design, reproducibility, and assay characteristics enabling discovery of kallikrein gene products for prostate disease diagnostics , 2005 .

[42]  Thomas Laurell,et al.  Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates. , 2005, Analytical biochemistry.

[43]  Xiao-Yang Zhu,et al.  Enzymatic activity on a chip: The critical role of protein orientation , 2005, Proteomics.

[44]  Bong Jin Hong,et al.  DNA microarrays on nanoscale-controlled surface , 2005, Nucleic acids research.

[45]  H. Lilja,et al.  Macro/nao-structured silicon as solid support for antibody arrays , 2005 .

[46]  Y. Berdichevsky,et al.  Versatile protein microarray based on carbohydrate‐binding modules , 2005, Proteomics.

[47]  F. Chen,et al.  Lithographic techniques and surface chemistries for the fabrication of PEG-passivated protein microarrays. , 2006, Biosensors & bioelectronics.

[48]  Igor Goychuk,et al.  Optimal Design of Microarray Immunoassays to Compensate for Kinetic Limitations , 2006, Molecular & Cellular Proteomics.

[49]  D. Daly,et al.  ELISA microarray technology as a high-throughput system for cancer biomarker validation , 2006, Expert review of proteomics.

[50]  Igor Goychuk,et al.  Kinetics of antigen binding to antibody microspots: Strong limitation by mass transport to the surface , 2006, Proteomics.

[51]  Jizhong Zhou,et al.  Protein microarrays on hybrid polymeric thin films prepared by self‐assembly of polyelectrolytes for multiple‐protein immunoassays , 2006, Proteomics.

[52]  Igor Goychuk,et al.  Antibody microarrays: the crucial impact of mass transport on assay kinetics and sensitivity , 2006, Expert review of molecular diagnostics.

[53]  B. Geierstanger,et al.  Antibody microarrays using resonance light-scattering particles for detection. , 2006, Methods in molecular biology.

[54]  Michael J Taussig,et al.  Improved affinity coupling for antibody microarrays: Engineering of double‐(His)6‐tagged single framework recombinant antibody fragments , 2006, Proteomics.

[55]  Bong Jin Hong,et al.  Sensitivity enhancement of DNA microarray on nano-scale controlled surface by using a streptavidin-fluorophore conjugate. , 2007, Biosensors & bioelectronics.